Discovery of an antifreeze protein in the leaves of Ammopiptanthus nanus

CORC > 新疆生态与地理研究所 > 中国科学院新疆生态与地理研究所 > 中国科学院新疆生态与地理研究所(2010年以前数据)

	Discovery of an antifreeze protein in the leaves of Ammopiptanthus nanus
	Yu ; Shanshan ; Yin ; Linke ; Mu ; Shuyong
刊名	CANADIAN JOURNAL OF PLANT SCIENCE
	2010
卷号	90 期号:1 页码:35-40
关键词	THERMAL HYSTERESIS PROTEIN PURIFICATION PLANT BACTERIA INSECTS
ISSN号	0008-4220
通讯作者	Yin, LK, Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Urumqi 830011, Peoples R China
中文摘要	Yu. S., Yin, L. and Mu, S. 2010. Discovery of an antifreeze protein in the leaves of Anitnopiptanthus nanus. Can. J. Plant Sci. 90: 35-40. Overwintering plants produce antifreeze proteins (AFPs) that allow plants to survive under freezing or subfreezing conditions. Ammopiptanthus nanus (M. Pop.) Cheng f. grows in the desert of Xinjiang., P.R. China, and our objective was to determine if its survival was dependent on AFP and to identify the protein. Using anion exchange and gel filtration, the AFP was extracted, isolated and purified from cold-acclimated A. nanus leaves. The thermal hysteresis activity (THA) of the antifreeze proteins was measured by differential scanning calorimetry. The THA of the AFP was 0.46 degrees C when the concentration of the protein was 20 mg mL(-1). The molecular weight of a band was about 119.24 kDa in SDS-PAGE gel. We detected (P < 0.05) the ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit by analyzing the 119.24 kDa protein using the Waters SYNAPT (TM) high definition mass spectrometry (HDMS (TM)) System. Our results indicate that there is an AFP in A. nanus leaves, and that it may play a vital role as a defence against the cold climate, thus increasing the chances for survival of A. nanus in its desert habitat.
学科主题	细胞生物学 ; 生物化学与分子生物学
收录类别	SCI
公开日期	2011-08-19
内容类型	期刊论文
源URL	[http://ir.xjlas.org/handle/365004/9998]
专题	新疆生态与地理研究所_中国科学院新疆生态与地理研究所(2010年以前数据)
推荐引用方式 GB/T 7714	Yu,Shanshan,Yin,et al. Discovery of an antifreeze protein in the leaves of Ammopiptanthus nanus[J]. CANADIAN JOURNAL OF PLANT SCIENCE,2010,90(1):35-40.
APA	Yu,Shanshan,Yin,Linke,Mu,&Shuyong.(2010).Discovery of an antifreeze protein in the leaves of Ammopiptanthus nanus.CANADIAN JOURNAL OF PLANT SCIENCE,90(1),35-40.
MLA	Yu,et al."Discovery of an antifreeze protein in the leaves of Ammopiptanthus nanus".CANADIAN JOURNAL OF PLANT SCIENCE 90.1(2010):35-40.