Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique
	Pei, De-Sheng; Sun, Yong-Hua; Long, Yong; Zhu, Zuo-Yan
刊名	MOLECULAR BIOLOGY REPORTS
	2008-06-01
卷号	35 期号:2 页码:139-143
关键词	external guide sequence no tail RNase P zebrafish
ISSN号	0301-4851
通讯作者	Sun, YH, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China
中文摘要	External guide sequence (EGS) technique, a branch of ribozyme strategy, can be enticed to cleave the target mRNA by forming a tRNA-like structure. In the present study, no tail gene (ntl), a key gene participating in the formation of normal tail, was used as a target for ribonuclease (RNase) P-mediated gene disruption in zebrafish in vivo. Transient expression of pH1-m3/4 ntl-EGS or pH1-3/4 ntl-EGS produced the full no tail phenotype at long-pec stage in proportion as 24 or 35%, respectively. As is expected that the full-length ntl mRNA of embryos at 50% epiboly stage decreased relative to control when injected the embryos with 3/4 EGS or m3/4 EGS RNA. Interestingly, ntl RNA transcripts, including the cleaved by EGS and the untouched, increased. Taken together, these results indicate that EGS strategy can work in zebrafish in vivo and becomes a potential tool for degradation of targeted mRNAs.
英文摘要	External guide sequence (EGS) technique, a branch of ribozyme strategy, can be enticed to cleave the target mRNA by forming a tRNA-like structure. In the present study, no tail gene (ntl), a key gene participating in the formation of normal tail, was used as a target for ribonuclease (RNase) P-mediated gene disruption in zebrafish in vivo. Transient expression of pH1-m3/4 ntl-EGS or pH1-3/4 ntl-EGS produced the full no tail phenotype at long-pec stage in proportion as 24 or 35%, respectively. As is expected that the full-length ntl mRNA of embryos at 50% epiboly stage decreased relative to control when injected the embryos with 3/4 EGS or m3/4 EGS RNA. Interestingly, ntl RNA transcripts, including the cleaved by EGS and the untouched, increased. Taken together, these results indicate that EGS strategy can work in zebrafish in vivo and becomes a potential tool for degradation of targeted mRNAs.
学科主题	Biochemistry & Molecular Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	RNASE-P ; RIBONUCLEASE P ; MODEL ; CLEAVAGE ; SIRNAS ; ASTHMA ; CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000256082300009
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8114]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Pei, De-Sheng,Sun, Yong-Hua,Long, Yong,et al. Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique[J]. MOLECULAR BIOLOGY REPORTS,2008,35(2):139-143.
APA	Pei, De-Sheng,Sun, Yong-Hua,Long, Yong,&Zhu, Zuo-Yan.(2008).Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique.MOLECULAR BIOLOGY REPORTS,35(2),139-143.
MLA	Pei, De-Sheng,et al."Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique".MOLECULAR BIOLOGY REPORTS 35.2(2008):139-143.