Detection of cutaneous antibodies in excised skin explants from grass carp, Ctenopharyngodon idella (Valenciennes), immune to Scophthalmus maximus rhabdovirus

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Detection of cutaneous antibodies in excised skin explants from grass carp, Ctenopharyngodon idella (Valenciennes), immune to Scophthalmus maximus rhabdovirus
	Lue, A-J 1,2; Li, Z-Q 1; Zhang, Q-Y 1
刊名	JOURNAL OF FISH DISEASES
	2008-08-01
卷号	31 期号:8 页码:559-565
关键词	Ctenopharyngodon idellus cutaneous antibodies detection grass carp Scophthalmus maximus rhabdovirus skin culture
ISSN号	0140-7775
通讯作者	Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	This study determined whether cutaneous antibodies were present in excised skin explants of grass carp, Ctenopharyngodon idella, immune to Scophthalmus maximus rhabdovirus (SMRV). Culture fluid from immune skin explants were assayed by indirect enzyme-linked immunosorbent assay (iELISA), Western blot, indirect immunofluorescent assay (IFA) and flow cytometry (FCM). iELISA showed that cutaneous antibody titres were much lower (1:12) than antiserum titres (1:1458) from intraperitoneally immunized grass carp. The phosphoprotein and matrix protein antigens of purified SMRV proteins were recognized by cutaneous antibodies from skin culture fluid using Western blot. The skin culture fluid produced staining signals in viral assembly sites and cytoplasm of SMRV-infected epithelioma papulosum cyprini (EPC) cells by IFA. FCM showed that 4.39% SMRV-infected EPC cells were detected, while non-specific reaction was seen in 2% of control cells. This is the first description of cutaneous antibodies against SMRV in grass carp.
英文摘要	This study determined whether cutaneous antibodies were present in excised skin explants of grass carp, Ctenopharyngodon idella, immune to Scophthalmus maximus rhabdovirus (SMRV). Culture fluid from immune skin explants were assayed by indirect enzyme-linked immunosorbent assay (iELISA), Western blot, indirect immunofluorescent assay (IFA) and flow cytometry (FCM). iELISA showed that cutaneous antibody titres were much lower (1:12) than antiserum titres (1:1458) from intraperitoneally immunized grass carp. The phosphoprotein and matrix protein antigens of purified SMRV proteins were recognized by cutaneous antibodies from skin culture fluid using Western blot. The skin culture fluid produced staining signals in viral assembly sites and cytoplasm of SMRV-infected epithelioma papulosum cyprini (EPC) cells by IFA. FCM showed that 4.39% SMRV-infected EPC cells were detected, while non-specific reaction was seen in 2% of control cells. This is the first description of cutaneous antibodies against SMRV in grass carp.
学科主题	Fisheries; Marine & Freshwater Biology; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	ICTALURUS-PUNCTATUS RAFINESQUE ; CHANNEL CATFISH ; ICHTHYOPHTHIRIUS-MULTIFILIIS ; FLAVOBACTERIUM-COLUMNARE ; MARINE FISH ; L. ; MUCOSAL ; IMMUNIZATION ; APOPTOSIS ; RESPONSES
收录类别	SCI
语种	英语
WOS记录号	WOS:000257493600001
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8074]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Xuzhou Normal Univ, Sch Life Sci, Xuzhou, Peoples R China
推荐引用方式 GB/T 7714	Lue, A-J,Li, Z-Q,Zhang, Q-Y. Detection of cutaneous antibodies in excised skin explants from grass carp, Ctenopharyngodon idella (Valenciennes), immune to Scophthalmus maximus rhabdovirus[J]. JOURNAL OF FISH DISEASES,2008,31(8):559-565.
APA	Lue, A-J,Li, Z-Q,&Zhang, Q-Y.(2008).Detection of cutaneous antibodies in excised skin explants from grass carp, Ctenopharyngodon idella (Valenciennes), immune to Scophthalmus maximus rhabdovirus.JOURNAL OF FISH DISEASES,31(8),559-565.
MLA	Lue, A-J,et al."Detection of cutaneous antibodies in excised skin explants from grass carp, Ctenopharyngodon idella (Valenciennes), immune to Scophthalmus maximus rhabdovirus".JOURNAL OF FISH DISEASES 31.8(2008):559-565.