Molecular characterization and expression pattern of fetuin-B in gibel carp (Carassius auratus gibelio)

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Molecular characterization and expression pattern of fetuin-B in gibel carp (Carassius auratus gibelio)
	Liu, Jing-Xia; Zhai, Yan-Hua; Geng, Fan-Suo; Xia, Jian-Hong; Gui, Jian-Fang
刊名	BIOCHEMICAL GENETICS
	2008-10-01
卷号	46 期号:9-10 页码:620-633
关键词	fetuin-B embryogenesis organogenesis extra-hepatic transcription gibel carp
ISSN号	0006-2928
通讯作者	Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Fetuin-B has recently been cloned and identified from rats, mice, and humans; their expression patterns, however, have not been elucidated yet. In this study, Cagfetuin-B has been cloned in gibel carp. RT-PCR and Western blot detection revealed that Cagfetuin-B is first transcribed from the blastula stage and at a relatively stable level afterward during embryogenesis and the larval stage. Cagfetuin-B transcripts are predominantly distributed over the yolk syncytial layer in the early embryos and later restricted to the cells of liver and brain in newly hatched larvae. Moreover, a dynamic distribution of Cagfetuin-B protein was observed in brain, kidney, liver, and skin during morphogenesis. In adult fish, Cagfetuin-B transcripts are restricted in liver and ovary. Our work, for the first time, revealed the extra-hepatic transcription and a dynamic distribution of fetuin-B during embryogenesis and in adults, which indicates the potential roles of fetuin-B in fish organogenesis.
英文摘要	Fetuin-B has recently been cloned and identified from rats, mice, and humans; their expression patterns, however, have not been elucidated yet. In this study, Cagfetuin-B has been cloned in gibel carp. RT-PCR and Western blot detection revealed that Cagfetuin-B is first transcribed from the blastula stage and at a relatively stable level afterward during embryogenesis and the larval stage. Cagfetuin-B transcripts are predominantly distributed over the yolk syncytial layer in the early embryos and later restricted to the cells of liver and brain in newly hatched larvae. Moreover, a dynamic distribution of Cagfetuin-B protein was observed in brain, kidney, liver, and skin during morphogenesis. In adult fish, Cagfetuin-B transcripts are restricted in liver and ovary. Our work, for the first time, revealed the extra-hepatic transcription and a dynamic distribution of fetuin-B during embryogenesis and in adults, which indicates the potential roles of fetuin-B in fish organogenesis.
学科主题	Biochemistry & Molecular Biology; Genetics & Heredity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Genetics & Heredity
研究领域[WOS]	Biochemistry & Molecular Biology ; Genetics & Heredity
关键词[WOS]	SILVER CRUCIAN CARP ; RAT FETUIN ; GLYCOPROTEIN/FETUIN-A ; INSULIN-RECEPTOR ; TYROSINE KINASE ; PROTEIN ; GENE ; INHIBITOR ; FAMILY ; MEMBER
收录类别	SCI
资助信息	National Major Basic Research Program [2004CB117401]
语种	英语
WOS记录号	WOS:000259063800009
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8016]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jing-Xia,Zhai, Yan-Hua,Geng, Fan-Suo,et al. Molecular characterization and expression pattern of fetuin-B in gibel carp (Carassius auratus gibelio)[J]. BIOCHEMICAL GENETICS,2008,46(9-10):620-633.
APA	Liu, Jing-Xia,Zhai, Yan-Hua,Geng, Fan-Suo,Xia, Jian-Hong,&Gui, Jian-Fang.(2008).Molecular characterization and expression pattern of fetuin-B in gibel carp (Carassius auratus gibelio).BIOCHEMICAL GENETICS,46(9-10),620-633.
MLA	Liu, Jing-Xia,et al."Molecular characterization and expression pattern of fetuin-B in gibel carp (Carassius auratus gibelio)".BIOCHEMICAL GENETICS 46.9-10(2008):620-633.