Apo-14 is required for digestive system organogenesis during fish embryogenesis and larval development

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Apo-14 is required for digestive system organogenesis during fish embryogenesis and larval development
	Xia, Jian-Hong; Liu, Jing-Xia; Zhou, Li; Li, Zhi; Gui, Jian-Fang
刊名	INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
	2008
卷号	52 期号:8 页码:1089-1098
关键词	apolipoprotein apo-14 digestive system organogenesis gibel carp
ISSN号	0214-6282
通讯作者	Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Apo-14 is a fish-specific apolipoprotein and its biological function remains unknown. In this study, CagApo-14 was cloned from gibel carp (Carassius auratus gibelio) and its expression pattern was investigated during embryogenesis and early larval development. The CagApo-14 transcript and its protein product were firstly localized in the yolk syncytial layer at a high level during embryogenesis, and then found to be restricted to the digestive system including liver and intestine in later embryos and early larvae. Immunofluorescence staining in larvae and adults indicated that CagApo-14 protein was predominantly synthesized in and excreted from sinusoidal endothelial cells of liver tissue. Morpholino knockdown of CagApo-14 resulted in severe disruption of digestive organs including liver, intestine, pancreas and swim bladder. Moreover, yolk lipid transportation and utilization were severely affected in the CagApo-14 morphants. Overall, this data indicates that CagApo-14 is required for digestive system organogenesis during fish embryogenesis and larval development.
英文摘要	Apo-14 is a fish-specific apolipoprotein and its biological function remains unknown. In this study, CagApo-14 was cloned from gibel carp (Carassius auratus gibelio) and its expression pattern was investigated during embryogenesis and early larval development. The CagApo-14 transcript and its protein product were firstly localized in the yolk syncytial layer at a high level during embryogenesis, and then found to be restricted to the digestive system including liver and intestine in later embryos and early larvae. Immunofluorescence staining in larvae and adults indicated that CagApo-14 protein was predominantly synthesized in and excreted from sinusoidal endothelial cells of liver tissue. Morpholino knockdown of CagApo-14 resulted in severe disruption of digestive organs including liver, intestine, pancreas and swim bladder. Moreover, yolk lipid transportation and utilization were severely affected in the CagApo-14 morphants. Overall, this data indicates that CagApo-14 is required for digestive system organogenesis during fish embryogenesis and larval development.
学科主题	Developmental Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Developmental Biology
研究领域[WOS]	Developmental Biology
关键词[WOS]	AURATUS-GIBELIO BLOCH ; SILVER CRUCIAN CARP ; APOLIPOPROTEIN-A-I ; GENE-EXPRESSION ; EMBRYONIC-DEVELOPMENT ; CARASSIUS-AURATUS ; LIVER DEVELOPMENT ; LIPID NUTRITION ; GUT DEVELOPMENT ; ZEBRAFISH
收录类别	SCI
资助信息	National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [90408013]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]
语种	英语
WOS记录号	WOS:000262153300009
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7926]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Xia, Jian-Hong,Liu, Jing-Xia,Zhou, Li,et al. Apo-14 is required for digestive system organogenesis during fish embryogenesis and larval development[J]. INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY,2008,52(8):1089-1098.
APA	Xia, Jian-Hong,Liu, Jing-Xia,Zhou, Li,Li, Zhi,&Gui, Jian-Fang.(2008).Apo-14 is required for digestive system organogenesis during fish embryogenesis and larval development.INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY,52(8),1089-1098.
MLA	Xia, Jian-Hong,et al."Apo-14 is required for digestive system organogenesis during fish embryogenesis and larval development".INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY 52.8(2008):1089-1098.