Mouse Toxicity of Anabaena flos-aquae from Lake Dianchi, China

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Mouse Toxicity of Anabaena flos-aquae from Lake Dianchi, China
	Pan, Xiaojie 1,2; Chang, Fengyi 1,2; Liu, Yongding 1; Li, Dunhai 1; Xu, Aihua 1,2; Shen, Yinwu 1; Huang, Zebo 3
刊名	ENVIRONMENTAL TOXICOLOGY
	2009-02-01
卷号	24 期号:1 页码:10-18
关键词	toxicity Anabaena flos-aquae mouse bioassay biochemical parameters histopathology Lake Dianchi
ISSN号	1520-4081
通讯作者	Liu, YD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	Some species of the genera Anabaena can produce various kinds of cyanotoxins, which may pose risks to environment and human health. Anabaena has frequently been observed in eutrophic freshwater of China in recent years, but its toxicity has been reported only in a few studies. In the present study, the toxicity of an Anabaena flos-aquae strain isolated from Lake Dianchi was investigated. Acute toxicity testing was performed by mouse bioassay using crude extracts from the lyophilized cultures. The mice exposed to crude extracts showed visible symptoms of toxicity and died within 10-24 h of the injection. Serum biochemical parameters were evaluated by the use of commercial diagnostic kits. Significant alterations were found in the serum biochemical parameters: alkaline phosphatase (AKP), gamma-glutamyl transpepticlase (gamma-GT), aspartate amino transferase (AST), alanine amino transferase (ALT), AST/ALT ratio, total protein content, albumin content, albumin/globulin (A/G) ratio, blood urea nitrogen (BUN), serum creatinine (Ssr), and total antioxidative capacity (T-AOC). Histopathological observations were carried out with hematoxylin and eosin (HE) stain under light microscope. Severe lesions were seen in the livers, kidneys, and lungs of the mice injected with crude extracts. The alterations of biochemical parameters were in a dose-dependent manner, and the severities of histological lesions were in the same manner. Based on biochemical and histological studies, this research firstly shows the presence of toxin-producing Anabaena species in Lake Dianchi and the toxic effects of its crude extracts on mammals. (C) 2008 Wiley Periodicals, Inc. Environ Toxicol 24: 10-18, 2009.
英文摘要	Some species of the genera Anabaena can produce various kinds of cyanotoxins, which may pose risks to environment and human health. Anabaena has frequently been observed in eutrophic freshwater of China in recent years, but its toxicity has been reported only in a few studies. In the present study, the toxicity of an Anabaena flos-aquae strain isolated from Lake Dianchi was investigated. Acute toxicity testing was performed by mouse bioassay using crude extracts from the lyophilized cultures. The mice exposed to crude extracts showed visible symptoms of toxicity and died within 10-24 h of the injection. Serum biochemical parameters were evaluated by the use of commercial diagnostic kits. Significant alterations were found in the serum biochemical parameters: alkaline phosphatase (AKP), gamma-glutamyl transpepticlase (gamma-GT), aspartate amino transferase (AST), alanine amino transferase (ALT), AST/ALT ratio, total protein content, albumin content, albumin/globulin (A/G) ratio, blood urea nitrogen (BUN), serum creatinine (Ssr), and total antioxidative capacity (T-AOC). Histopathological observations were carried out with hematoxylin and eosin (HE) stain under light microscope. Severe lesions were seen in the livers, kidneys, and lungs of the mice injected with crude extracts. The alterations of biochemical parameters were in a dose-dependent manner, and the severities of histological lesions were in the same manner. Based on biochemical and histological studies, this research firstly shows the presence of toxin-producing Anabaena species in Lake Dianchi and the toxic effects of its crude extracts on mammals. (C) 2008 Wiley Periodicals, Inc. Environ Toxicol 24: 10-18, 2009.
学科主题	Environmental Sciences; Toxicology; Water Resources
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Environmental Sciences ; Toxicology ; Water Resources
研究领域[WOS]	Environmental Sciences & Ecology ; Toxicology ; Water Resources
关键词[WOS]	CYLINDROSPERMOPSIS-RACIBORSKII ; TOXIN ; PHORMIDIUM ; STRAINS ; BLOOMS ; LR
收录类别	SCI
资助信息	Ministry of Science and Technology, People's Republic of China [2002CB412300, 2005AA60101504]; Chinese Academy of Sciences [KZCX2-YW-426, KSCX2-1-10]
语种	英语
WOS记录号	WOS:000263533000002
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7862]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Wuhan Univ, Coll Pharm, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Pan, Xiaojie,Chang, Fengyi,Liu, Yongding,et al. Mouse Toxicity of Anabaena flos-aquae from Lake Dianchi, China[J]. ENVIRONMENTAL TOXICOLOGY,2009,24(1):10-18.
APA	Pan, Xiaojie.,Chang, Fengyi.,Liu, Yongding.,Li, Dunhai.,Xu, Aihua.,...&Huang, Zebo.(2009).Mouse Toxicity of Anabaena flos-aquae from Lake Dianchi, China.ENVIRONMENTAL TOXICOLOGY,24(1),10-18.
MLA	Pan, Xiaojie,et al."Mouse Toxicity of Anabaena flos-aquae from Lake Dianchi, China".ENVIRONMENTAL TOXICOLOGY 24.1(2009):10-18.