Isolation and characterization of Argonaute 2: A key gene of the RNA interference pathway in the rare minnow, Gobiocypris rarus

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	Isolation and characterization of Argonaute 2: A key gene of the RNA interference pathway in the rare minnow, Gobiocypris rarus
	Su, Jianguo 1,2; Zhu, Zuoyan 1; Wang, Yaping 1; Jang, Songhun 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2009
卷号	26 期号:1 页码:164-170
关键词	Rare minnow (Gobiocypris rarus) Argonaute 2 RNA interference Gene cloning mRNA expression
ISSN号	1050-4648
通讯作者	Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Argonaute 2 gene plays a pivotal role in RNAi in many species. Herein is the first report of the cloning and characterization of Argonaute 2 gene in fish. The full-length cDNA of Gobiocypris rarus Argonaute 2 (GrAgo2) consisted of 3073 nucleotides encoding 869 amino acid residues with a calculated molecular weight of 98.499 kDa and an estimated isoelectric point of 9.18. Analysis of the deduced amino acid sequence showed the presence of two signature domains, PAZ and Piwi. RT-PCR analysis indicated that GrAgo2 mRNA expression could be detected in widespread tissues. After infection with grass carp reovirus, GrAgo2 expression was up-regulated from 12 h post-injection (p < 0.05) and returned to control levels at 48 h post-injection (p > 0.05). These data imply that GrAgo2 is involved in antiviral defense in rare minnow. (C) 2008 Published by Elsevier Ltd.
英文摘要	Argonaute 2 gene plays a pivotal role in RNAi in many species. Herein is the first report of the cloning and characterization of Argonaute 2 gene in fish. The full-length cDNA of Gobiocypris rarus Argonaute 2 (GrAgo2) consisted of 3073 nucleotides encoding 869 amino acid residues with a calculated molecular weight of 98.499 kDa and an estimated isoelectric point of 9.18. Analysis of the deduced amino acid sequence showed the presence of two signature domains, PAZ and Piwi. RT-PCR analysis indicated that GrAgo2 mRNA expression could be detected in widespread tissues. After infection with grass carp reovirus, GrAgo2 expression was up-regulated from 12 h post-injection (p < 0.05) and returned to control levels at 48 h post-injection (p > 0.05). These data imply that GrAgo2 is involved in antiviral defense in rare minnow. (C) 2008 Published by Elsevier Ltd.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	FAMILY ; EXPRESSION ; IDENTIFICATION ; PROTEINS ; DOMAINS ; COMPLEX
收录类别	SCI
资助信息	National Natural Science Foundation of China [30740009, 30871917, 30540084]; National Basic Research Program of China [2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; Northwest A&F University in China [01140309, 05ZR096, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology, CAS [2007171309]
语种	英语
WOS记录号	WOS:000264015700020
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7834]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.NW A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China
推荐引用方式 GB/T 7714	Su, Jianguo,Zhu, Zuoyan,Wang, Yaping,et al. Isolation and characterization of Argonaute 2: A key gene of the RNA interference pathway in the rare minnow, Gobiocypris rarus[J]. FISH & SHELLFISH IMMUNOLOGY,2009,26(1):164-170.
APA	Su, Jianguo,Zhu, Zuoyan,Wang, Yaping,&Jang, Songhun.(2009).Isolation and characterization of Argonaute 2: A key gene of the RNA interference pathway in the rare minnow, Gobiocypris rarus.FISH & SHELLFISH IMMUNOLOGY,26(1),164-170.
MLA	Su, Jianguo,et al."Isolation and characterization of Argonaute 2: A key gene of the RNA interference pathway in the rare minnow, Gobiocypris rarus".FISH & SHELLFISH IMMUNOLOGY 26.1(2009):164-170.