Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus
	Su, Jianguo 1,2; Zhu, Zuoyan 1; Wang, Yaping 1; Zou, Jun 3; Wang, Na 1; Jang, Songhun 1
刊名	AQUACULTURE
	2009-04-03
卷号	289 期号:1-2 页码:1-5
关键词	Gobiocypris torus Grass carp reovirus RNA interference Dicer mRNA expression Virus inclusion body
ISSN号	0044-8486
通讯作者	Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 S Donghu Rd, Wuhan 430072, Peoples R China
中文摘要	Dicer catalyzes the initiation step of RNA interference (RNAi) which is known to play a significant role in innate immune response to viral infection in many organisms. To study the RNAi-related pathway after virus infection in fish, we identified a partial cDNA sequence of dicer from rare minnow, Gobiocypris rants. Real-time quantitative RT-PCR (qRT-PCR) demonstrated the Dicer transcript level was the highest at zygote stage, decreased at prim-5 stage, and was stable from the protruding mouth to adult stage. Regular RT-PCR analysis showed that the Dicer gene expressed widely in the tested tissues, including brain, gill, heart, intestine, kidney, liver, muscle, ovary, spleen and testis. The expression of Dicer mRNA was significantly increased in the early period of Grass carp reovirus (GCRV) infection, and declined from 24 It post-injection (h p.i.) (P<0.05). The mRNA expression returned to control levels at 48 h p.i. (P>0.05). Under transmission electron microscope, virions were difficulty to find out in 12 h p.i., and virus inclusion bodies and few scattered viral particles were easily visualized from 24 h p.i. to moribund. These results implied GCRV triggered the RNAi pathway in the early stages of infection and perhaps virus inclusion bodies suppressed the antiviral functions of RNAi mechanism. (C) 2009 Published by Elsevier B.V.
英文摘要	Dicer catalyzes the initiation step of RNA interference (RNAi) which is known to play a significant role in innate immune response to viral infection in many organisms. To study the RNAi-related pathway after virus infection in fish, we identified a partial cDNA sequence of dicer from rare minnow, Gobiocypris rants. Real-time quantitative RT-PCR (qRT-PCR) demonstrated the Dicer transcript level was the highest at zygote stage, decreased at prim-5 stage, and was stable from the protruding mouth to adult stage. Regular RT-PCR analysis showed that the Dicer gene expressed widely in the tested tissues, including brain, gill, heart, intestine, kidney, liver, muscle, ovary, spleen and testis. The expression of Dicer mRNA was significantly increased in the early period of Grass carp reovirus (GCRV) infection, and declined from 24 It post-injection (h p.i.) (P<0.05). The mRNA expression returned to control levels at 48 h p.i. (P>0.05). Under transmission electron microscope, virions were difficulty to find out in 12 h p.i., and virus inclusion bodies and few scattered viral particles were easily visualized from 24 h p.i. to moribund. These results implied GCRV triggered the RNAi pathway in the early stages of infection and perhaps virus inclusion bodies suppressed the antiviral functions of RNAi mechanism. (C) 2009 Published by Elsevier B.V.
学科主题	Fisheries; Marine & Freshwater Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Marine & Freshwater Biology
研究领域[WOS]	Fisheries ; Marine & Freshwater Biology
关键词[WOS]	INTERFERENCE PATHWAY ; DICER ; VIRUSES ; MORPHOGENESIS ; DEGRADATION ; TRANSCRIPTS ; DROSOPHILA ; PROTEINS ; DOMAIN ; DSRNA
收录类别	SCI
资助信息	National Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298]
语种	英语
WOS记录号	WOS:000264942400001
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7792]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Coll Anim Sci & Technol, Yangling 712100, Peoples R China 3.Univ Aberdeen, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland
推荐引用方式 GB/T 7714	Su, Jianguo,Zhu, Zuoyan,Wang, Yaping,et al. Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus[J]. AQUACULTURE,2009,289(1-2):1-5.
APA	Su, Jianguo,Zhu, Zuoyan,Wang, Yaping,Zou, Jun,Wang, Na,&Jang, Songhun.(2009).Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus.AQUACULTURE,289(1-2),1-5.
MLA	Su, Jianguo,et al."Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus".AQUACULTURE 289.1-2(2009):1-5.