Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus)

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus)
	Su, Jianguo 1,2; Yang, Chunrong 2; Zhu, Zuoyan 1; Wang, Yaping 1; Jang, Songhun 1; Liao, Lanjie 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2009-06-01
卷号	26 期号:6 页码:828-835
关键词	Rare minnow (Gobiocypris rarus) Mx gene Grass carp reovirus Transgenic fish Antiviral activity Gene cloning
ISSN号	1050-4648
通讯作者	Wang, YP, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	In the interferon-induced antiviral mechanisms, the Mx pathway is one of the most powerful. Mx proteins have direct antiviral activity and inhibit a wide range of viruses by blocking an early stage of the viral genome replication cycle. However, antiviral activity of piscine Mx remains unclear in vivo. In the present study, an Mx-like gene was cloned, characterized and gene-transferred in rare minnow Gobiocypris rarus, and its antiviral activity was confirmed in vivo. The full length of the rare minnow Mx-like cDNA is 2241 bp in length and encodes a polypeptide of 625 amino acids with an estimated molecular mass of 70.928 kDa and a predicted isoelectric point of 7.33. Analysis of the deduced amino acid sequence indicated that the mature peptide contains an amino-terminal tripartite GTP-binding motif, a dynamin family signature sequence, a GTPase effector domain and two carboxy-terminal leucine zipper motifs, and is the most similar to the crucian carp (Carassius auratus) Mx3 sequence with an identity of 89%. Both P0 and F1 generations of Mx-transgenic rare minnow demonstrated very significantly high survival rate to GCRV infection (P < 0.01). The mRNA expression of Mx gene was consistent with survival rate in F1 generation. The virus yield was also concurrent with survival time using electron microscope technology. Rare minnow has Mx gene(s) of its own but introducing more Mx gene improves their resistance to GCRV. Mx-transgenic rare minnow might contribute to control the GCRV diseases. (C) 2008 Published by Elsevier Ltd.
英文摘要	In the interferon-induced antiviral mechanisms, the Mx pathway is one of the most powerful. Mx proteins have direct antiviral activity and inhibit a wide range of viruses by blocking an early stage of the viral genome replication cycle. However, antiviral activity of piscine Mx remains unclear in vivo. In the present study, an Mx-like gene was cloned, characterized and gene-transferred in rare minnow Gobiocypris rarus, and its antiviral activity was confirmed in vivo. The full length of the rare minnow Mx-like cDNA is 2241 bp in length and encodes a polypeptide of 625 amino acids with an estimated molecular mass of 70.928 kDa and a predicted isoelectric point of 7.33. Analysis of the deduced amino acid sequence indicated that the mature peptide contains an amino-terminal tripartite GTP-binding motif, a dynamin family signature sequence, a GTPase effector domain and two carboxy-terminal leucine zipper motifs, and is the most similar to the crucian carp (Carassius auratus) Mx3 sequence with an identity of 89%. Both P0 and F1 generations of Mx-transgenic rare minnow demonstrated very significantly high survival rate to GCRV infection (P < 0.01). The mRNA expression of Mx gene was consistent with survival rate in F1 generation. The virus yield was also concurrent with survival time using electron microscope technology. Rare minnow has Mx gene(s) of its own but introducing more Mx gene improves their resistance to GCRV. Mx-transgenic rare minnow might contribute to control the GCRV diseases. (C) 2008 Published by Elsevier Ltd.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	VESICULAR STOMATITIS-VIRUS ; IN-VITRO INHIBITION ; INFLUENZA-VIRUS ; ANTIVIRAL ACTIVITY ; ATLANTIC SALMON ; EXPRESSION ANALYSIS ; SOLE AQUABIRNAVIRUS ; CELL-LINES ; PROTEIN ; GENE
收录类别	SCI
资助信息	National Natural Science Foundation of China [30740009, 30871917]; project-sponsored by SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology [2007FB09]
语种	英语
WOS记录号	WOS:000266965900002
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7708]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China
推荐引用方式 GB/T 7714	Su, Jianguo,Yang, Chunrong,Zhu, Zuoyan,et al. Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus)[J]. FISH & SHELLFISH IMMUNOLOGY,2009,26(6):828-835.
APA	Su, Jianguo,Yang, Chunrong,Zhu, Zuoyan,Wang, Yaping,Jang, Songhun,&Liao, Lanjie.(2009).Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus).FISH & SHELLFISH IMMUNOLOGY,26(6),828-835.
MLA	Su, Jianguo,et al."Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus)".FISH & SHELLFISH IMMUNOLOGY 26.6(2009):828-835.