The search for the IFN-gamma receptor in fish: Functional and expression analysis of putative binding and signalling chains in rainbow trout Oncorhynchus mykiss

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	The search for the IFN-gamma receptor in fish: Functional and expression analysis of putative binding and signalling chains in rainbow trout Oncorhynchus mykiss
	Gao, Q.1,2; Nie, P.2; Thompson, K. D.3; Adams, A.; Wang, T.1; Secombes, C. J.1; Zou, J.1
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2009-08-01
卷号	33 期号:8 页码:920-931
关键词	IFN-gamma receptor Rainbow trout Characterization Expression analysis
ISSN号	0145-305X
通讯作者	Zou, J, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland
中文摘要	Interferons (IFNs), consisting of three major subfamilies, type I, type II (gamma) and type III (lambda) IFN, activate vertebrate antiviral defences once bound to their receptors. The three IFN subfamilies bind to different receptors, IFNAR1 and IFNAR2 for type I IFNs, IFN gamma R1 and IFN gamma R2 for type II IFN, and IL-28R1 and IL-10R2 for type III IFNs. In fish, although many types I and II IFN genes have been cloned, little is known about their receptors. In this report, two putative IFN-gamma receptor chains were identified and sequenced in rainbow trout (Oncorhynchus mykiss), and found to have many common characteristics with mammalian type II IFN receptor family members. The presented gene synteny analysis, phylogenetic tree analysis and ligand binding analysis all suggest that these molecules are the authentic IFN gamma Rs in fish. They are widely expressed in tissues, with IFN gamma R1 typically more highly expressed than IFN gamma R2. Using the trout RTG-2 cell line it was possible to show that the individual chains could be differentially modulated, with rIFN-gamma and rIL-1 beta down regulating IFN gamma R1 expression but up regulating IFN gamma R2 expression. Overexpression of the two receptor chains in RTG-2 cells revealed that the level of IFN gamma R2 transcript was crucial for responsiveness to rIFN-gamma, in terms of inducing gamma IP expression. Transfection experiments showed that the two putative receptors specifically bound to rIFN-gamma. These findings are discussed in the context of how the IFN gamma R may bind IFN-gamma in fish and the importance of the individual receptor chains to signal transduction. (c) 2009 Elsevier Ltd. All rights reserved.
英文摘要	Interferons (IFNs), consisting of three major subfamilies, type I, type II (gamma) and type III (lambda) IFN, activate vertebrate antiviral defences once bound to their receptors. The three IFN subfamilies bind to different receptors, IFNAR1 and IFNAR2 for type I IFNs, IFN gamma R1 and IFN gamma R2 for type II IFN, and IL-28R1 and IL-10R2 for type III IFNs. In fish, although many types I and II IFN genes have been cloned, little is known about their receptors. In this report, two putative IFN-gamma receptor chains were identified and sequenced in rainbow trout (Oncorhynchus mykiss), and found to have many common characteristics with mammalian type II IFN receptor family members. The presented gene synteny analysis, phylogenetic tree analysis and ligand binding analysis all suggest that these molecules are the authentic IFN gamma Rs in fish. They are widely expressed in tissues, with IFN gamma R1 typically more highly expressed than IFN gamma R2. Using the trout RTG-2 cell line it was possible to show that the individual chains could be differentially modulated, with rIFN-gamma and rIL-1 beta down regulating IFN gamma R1 expression but up regulating IFN gamma R2 expression. Overexpression of the two receptor chains in RTG-2 cells revealed that the level of IFN gamma R2 transcript was crucial for responsiveness to rIFN-gamma, in terms of inducing gamma IP expression. Transfection experiments showed that the two putative receptors specifically bound to rIFN-gamma. These findings are discussed in the context of how the IFN gamma R may bind IFN-gamma in fish and the importance of the individual receptor chains to signal transduction. (c) 2009 Elsevier Ltd. All rights reserved.
学科主题	Immunology; Zoology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Immunology ; Zoology
研究领域[WOS]	Immunology ; Zoology
关键词[WOS]	ART. NO. 29 ; BETA-CHAIN ; CELL-LINE ; IDENTIFICATION ; GENE ; TUBERCULOSIS ; VERTEBRATES ; MODULATION ; ZEBRAFISH ; EVOLUTION
收录类别	SCI
资助信息	Royal Society International Incoming Fellowship ; K C Wang Education Foundation ; Chinese Academy of Sciences ; BBSRC [BB/C506021/1]
语种	英语
WOS记录号	WOS:000267004700005
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7686]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.Univ Stirling, Inst Aquaculture, Stirling FP9 4LA, Scotland
推荐引用方式 GB/T 7714	Gao, Q.,Nie, P.,Thompson, K. D.,et al. The search for the IFN-gamma receptor in fish: Functional and expression analysis of putative binding and signalling chains in rainbow trout Oncorhynchus mykiss[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2009,33(8):920-931.
APA	Gao, Q..,Nie, P..,Thompson, K. D..,Adams, A..,Wang, T..,...&Zou, J..(2009).The search for the IFN-gamma receptor in fish: Functional and expression analysis of putative binding and signalling chains in rainbow trout Oncorhynchus mykiss.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,33(8),920-931.
MLA	Gao, Q.,et al."The search for the IFN-gamma receptor in fish: Functional and expression analysis of putative binding and signalling chains in rainbow trout Oncorhynchus mykiss".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 33.8(2009):920-931.