Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella)

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	Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella)
	Su, Jianguo 1; Jang, Songhun 2; Yang, Chunrong 1; Wang, Yaping 2; Zhu, Zuoyan 2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2009-09-01
卷号	27 期号:3 页码:433-439
关键词	Ctenopharyngodon idella Toll-like receptor 3 Genomic organization Gene cloning Grass carp reovirus
ISSN号	1050-4648
通讯作者	Su, JG, NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China
中文摘要	Toll-like receptor 3 (TLR3) participates in the innate immune response by recognizing viral pathogens. To investigate grass carp immune system responding to GCRV (grass carp reovirus) infection, the full-length cDNA sequence and genomic organization of grass carp TLR3 (CiTLR3) was identified and characterized. The full-length genome sequence of CiTLR3 is composed of 5668 nucleotides, including five exons and four introns. The full-length of CiTLR3 cDNA is 3681 bp in length and encodes a polypeptide of 904 amino acids with an estimated molecular mass of 102,765 Da and a predicted isoelectric point of 8.35. Analysis of the deduced amino acid sequence indicated that CiTLR3 has four main structural domains, including a signal peptide sequence, 14 LRR (leucine-rich repeat) motifs, a transmembrane region and a TIR (Toll/interleukin-1 receptor) domain. It is most similar to the crucian carp (Carassius auratus) TLR3 amino acid sequence with an identity of 99%. Quantitative RT-PCR analysis showed that CiTLR3 transcripts were significantly up-regulated starting at day 1 and continued through day 7 following GCRV infection (P < 0.05). These data implied that CiTLR3 is involved in antiviral defense, provide molecular and functional information for grass carp TLR3, and implicate their role in mediating immune protection against grass carp viral diseases. (C) 2009 Elsevier Ltd. All rights reserved.
英文摘要	Toll-like receptor 3 (TLR3) participates in the innate immune response by recognizing viral pathogens. To investigate grass carp immune system responding to GCRV (grass carp reovirus) infection, the full-length cDNA sequence and genomic organization of grass carp TLR3 (CiTLR3) was identified and characterized. The full-length genome sequence of CiTLR3 is composed of 5668 nucleotides, including five exons and four introns. The full-length of CiTLR3 cDNA is 3681 bp in length and encodes a polypeptide of 904 amino acids with an estimated molecular mass of 102,765 Da and a predicted isoelectric point of 8.35. Analysis of the deduced amino acid sequence indicated that CiTLR3 has four main structural domains, including a signal peptide sequence, 14 LRR (leucine-rich repeat) motifs, a transmembrane region and a TIR (Toll/interleukin-1 receptor) domain. It is most similar to the crucian carp (Carassius auratus) TLR3 amino acid sequence with an identity of 99%. Quantitative RT-PCR analysis showed that CiTLR3 transcripts were significantly up-regulated starting at day 1 and continued through day 7 following GCRV infection (P < 0.05). These data implied that CiTLR3 is involved in antiviral defense, provide molecular and functional information for grass carp TLR3, and implicate their role in mediating immune protection against grass carp viral diseases. (C) 2009 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	TOLL-LIKE RECEPTOR-3 ; DOUBLE-STRANDED-RNA ; VIRAL-INFECTION ; DNA-SEQUENCES ; ACTIVATION ; TLR3 ; ZEBRAFISH ; IMMUNITY ; GENE
收录类别	SCI
资助信息	National Natural Science Foundation of China [30871917]; Program for New Century Excellent Talents in University [NCET-08-0466]; SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508]
语种	英语
WOS记录号	WOS:000269771000006
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7594]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Su, Jianguo,Jang, Songhun,Yang, Chunrong,et al. Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2009,27(3):433-439.
APA	Su, Jianguo,Jang, Songhun,Yang, Chunrong,Wang, Yaping,&Zhu, Zuoyan.(2009).Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,27(3),433-439.
MLA	Su, Jianguo,et al."Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 27.3(2009):433-439.