Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish

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	Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish
	Wu, Nan; Li, Chang-Jian; Gui, Jian-Fang
刊名	BIOCHEMICAL GENETICS
	2009-12-01
卷号	47 期号:11-12 页码:749-762
关键词	Oogenesis Spermatogenesis Sperm decondensation Carassius auratus gibelio Carassius auratus
ISSN号	0006-2928
通讯作者	Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated by immunodepletion and in vitro sperm decondensation experiments. The data suggest that the cloned nucleophosmin should share expressional and functional characterization with nucleoplasmin and therefore provide novel evidence for a functional commonality of nucleophosmin and nucleoplasmin in fish.
英文摘要	Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated by immunodepletion and in vitro sperm decondensation experiments. The data suggest that the cloned nucleophosmin should share expressional and functional characterization with nucleoplasmin and therefore provide novel evidence for a functional commonality of nucleophosmin and nucleoplasmin in fish.
学科主题	Biochemistry & Molecular Biology; Genetics & Heredity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Genetics & Heredity
研究领域[WOS]	Biochemistry & Molecular Biology ; Genetics & Heredity
关键词[WOS]	CARASSIUS-AURATUS-GIBELIO ; SILVER CRUCIAN CARP ; CHROMATIN DECONDENSATION ; SPERM DECONDENSATION ; XENOPUS-LAEVIS ; NUCLEOPLASMIN ; PROTEIN ; RNA ; NUCLEOPHOSMIN ; EXPRESSION
收录类别	SCI
资助信息	National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301]
语种	英语
WOS记录号	WOS:000272015400001
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7480]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Wu, Nan,Li, Chang-Jian,Gui, Jian-Fang. Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish[J]. BIOCHEMICAL GENETICS,2009,47(11-12):749-762.
APA	Wu, Nan,Li, Chang-Jian,&Gui, Jian-Fang.(2009).Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish.BIOCHEMICAL GENETICS,47(11-12),749-762.
MLA	Wu, Nan,et al."Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish".BIOCHEMICAL GENETICS 47.11-12(2009):749-762.