Evolutionary Conservation of Dazl Genomic Organization and its Continuous and Dynamic Distribution Throughout Germline Development in Gynogenetic Gibel Carp

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	Evolutionary Conservation of Dazl Genomic Organization and its Continuous and Dynamic Distribution Throughout Germline Development in Gynogenetic Gibel Carp
	Peng, Jin-Xia 1; Xie, Jian-Li 1; Zhou, Li 1; Hong, Yun-Han 1,2; Gui, Jian-Fang 1
刊名	JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
	2009-12-15
卷号	312B 期号:8 页码:855-871
关键词	CARASSIUS-AURATUS-GIBELIO SILVER CRUCIAN CARP XENOPUS OOCYTES MITOCHONDRIAL CLOUD RNA LOCALIZATION CELL DEVELOPMENT BINDING PROTEIN FAMILY PROTEINS VEGETAL CORTEX GENE ENCODES
ISSN号	1552-5007
通讯作者	Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, 7 Donghu S Rd, Wuhan 430072, Hubei, Peoples R China
中文摘要	To investigate germline development and germ cell specification, we identified a Dazl homolog (CagDazl) from gynogenetic gibel carp (Carassius auratus gibelio). Its cDNA sequence and BAC clone sequence analyses revealed the genomic organization conservation and conserved synteny of the Dazl family members and their neighborhood genes among vertebrates, especially in fish. Moreover, a polyclonal antibody specific to CagDazl was produced and used to examine its expression and distribution throughout germline development at protein level. Firstly, ovary-specific expression pattern of CagDazl was confirmed in adult tissues by RT-PCR and Western blot. In addition, in situ hybridization and immunofluorescence localization demonstrated its specific expression in germ cells, and both its transcript and protein were localized to germ plasm. Then, co-localization of CagDazl and mitochondrial cloud was found, confirming that CagDazl transcript and its protein are germ plasm component and move via METRO pathway during oogenesis. Furthermore, the CagDazl is abundant and continuous throughout germline development and germ cell specification including primordial germ cell (PGC) formation, oogonium differentiation, oocyte development, and embryogenesis, and the dynamic distribution occurs at different development stages. The data suggest that maternal CagDazl might play an important role in gibel carp PGC formation. Therefore, CagDazl is a useful and specific marker for tracing germ plasm and germ cell development in the gynogenetic gibel carp. In addition, in comparison with previous studies in sexual reproduction species, the continuous and dynamic distribution of CagDazl protein in the germ plasm throughout the life cycle seems to have significant implication in sex evolution of vertebrates. J. Exp. Zool. (Mol. Deu. Euol.) 312B:855-871, 2009. (C) 2009 Wiley-Liss, Inc.
英文摘要	To investigate germline development and germ cell specification, we identified a Dazl homolog (CagDazl) from gynogenetic gibel carp (Carassius auratus gibelio). Its cDNA sequence and BAC clone sequence analyses revealed the genomic organization conservation and conserved synteny of the Dazl family members and their neighborhood genes among vertebrates, especially in fish. Moreover, a polyclonal antibody specific to CagDazl was produced and used to examine its expression and distribution throughout germline development at protein level. Firstly, ovary-specific expression pattern of CagDazl was confirmed in adult tissues by RT-PCR and Western blot. In addition, in situ hybridization and immunofluorescence localization demonstrated its specific expression in germ cells, and both its transcript and protein were localized to germ plasm. Then, co-localization of CagDazl and mitochondrial cloud was found, confirming that CagDazl transcript and its protein are germ plasm component and move via METRO pathway during oogenesis. Furthermore, the CagDazl is abundant and continuous throughout germline development and germ cell specification including primordial germ cell (PGC) formation, oogonium differentiation, oocyte development, and embryogenesis, and the dynamic distribution occurs at different development stages. The data suggest that maternal CagDazl might play an important role in gibel carp PGC formation. Therefore, CagDazl is a useful and specific marker for tracing germ plasm and germ cell development in the gynogenetic gibel carp. In addition, in comparison with previous studies in sexual reproduction species, the continuous and dynamic distribution of CagDazl protein in the germ plasm throughout the life cycle seems to have significant implication in sex evolution of vertebrates. J. Exp. Zool. (Mol. Deu. Euol.) 312B:855-871, 2009. (C) 2009 Wiley-Liss, Inc.
学科主题	Evolutionary Biology; Developmental Biology; Zoology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Evolutionary Biology ; Developmental Biology ; Zoology
研究领域[WOS]	Evolutionary Biology ; Developmental Biology ; Zoology
关键词[WOS]	CARASSIUS-AURATUS-GIBELIO ; SILVER CRUCIAN CARP ; XENOPUS OOCYTES ; MITOCHONDRIAL CLOUD ; RNA LOCALIZATION ; CELL DEVELOPMENT ; BINDING PROTEIN ; FAMILY PROTEINS ; VEGETAL CORTEX ; GENE ENCODES
收录类别	SCI
资助信息	National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Chinese Academy of Sciences [KSCX2-YW-N-020, 075A011301, 085A021301]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ15]; Institute of Hydrobiology
语种	英语
WOS记录号	WOS:000271958800006
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7474]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Hubei, Peoples R China 2.Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore
推荐引用方式 GB/T 7714	Peng, Jin-Xia,Xie, Jian-Li,Zhou, Li,et al. Evolutionary Conservation of Dazl Genomic Organization and its Continuous and Dynamic Distribution Throughout Germline Development in Gynogenetic Gibel Carp[J]. JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION,2009,312B(8):855-871.
APA	Peng, Jin-Xia,Xie, Jian-Li,Zhou, Li,Hong, Yun-Han,&Gui, Jian-Fang.(2009).Evolutionary Conservation of Dazl Genomic Organization and its Continuous and Dynamic Distribution Throughout Germline Development in Gynogenetic Gibel Carp.JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION,312B(8),855-871.
MLA	Peng, Jin-Xia,et al."Evolutionary Conservation of Dazl Genomic Organization and its Continuous and Dynamic Distribution Throughout Germline Development in Gynogenetic Gibel Carp".JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION 312B.8(2009):855-871.