Solution structure of BmK alpha ITO1, an alpha-insect toxin from the venom of the Chinese scorpion Buthus martensii Karsch

CORC > 上海有机化学研究所 > 中国科学院上海有机化学研究所 > 上海有机化学研究所

	Solution structure of BmK alpha ITO1, an alpha-insect toxin from the venom of the Chinese scorpion Buthus martensii Karsch
	Tong XT(童孝田) ; Zhu J(朱静) ; Ma YG(马宇光) ; Chen X(陈翔) ; Wu G(吴宫) ; He FH(何发虎) ; Cao CY(曹春阳) ; Wu HM(吴厚铭)
刊名	Biochemistry
	2007
卷号	46 期号:40 页码:11322-11330
ISSN号	0006-2960
其他题名	马氏钳蝎毒液中抗昆虫毒素BmKaIT的溶液三维结构
通讯作者	吴厚铭
英文摘要	The solution structure of an alpha-insect toxin from Buthus martensii Karsch, BmK alpha IT01, has been determined by two-dimensional NMR spectroscopy and molecular modeling techniques. Combining the sequence homology comparison and toxicity bioassays, BmK alpha IT01 has been suggested to be a natural mutant of alpha-insect toxins and so can serve as a tool to study the relationship of structure-function among this group of toxins. The overall structure of BmK alpha IT01 shares a common core structure consisting of an a-helix packed against a three-stranded antiparallel beta-sheet, which exhibits distinctive local conformations within the loops connecting these secondary structure elements. The solution structure of BmKaIT01 features a non-proline cis peptide bond between Asn9 and Tyr10, which is proposed to mediate the spatial closing of the five-residue turn (Gln8-Cysl2) and the C-terminal segment (Arg58-His64) to form the NC domain and confer the toxin insect-specific bioactivity. Conformational heterogeneity is observed in the solution of BmK alpha IT01 and could be attributed to the cis-trans isomerization of the peptide bond between residues 9 and 10. The minor conformation of BmKaIT01 with a trans peptide bond between Asn9 and Tyr10 may be responsible for its moderate bioactivity against mammals. The cis-trans isomerization of the peptide bond between residues 9 and 10 may be the structural basis of dual pharmacological activities of a-insect and cc-like scorpion toxins, which is supported by the fact that conformational heterogeneity occurs in the solution structures of Lqh alpha IT, LqqIII, and LqhIII and by comparison of the solution structure of BmK alpha IT01 with those of some relevant alpha-type toxins.
学科主题	生命有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1021/bi7006788
语种	英语
WOS记录号	WOS:000249926300010
公开日期	2013-02-21
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/17379]
专题	上海有机化学研究所_上海有机化学研究所
推荐引用方式 GB/T 7714	Tong XT,Zhu J,Ma YG,et al. Solution structure of BmK alpha ITO1, an alpha-insect toxin from the venom of the Chinese scorpion Buthus martensii Karsch[J]. Biochemistry,2007,46(40):11322-11330.
APA	童孝田.,朱静.,马宇光.,陈翔.,吴宫.,...&吴厚铭.(2007).Solution structure of BmK alpha ITO1, an alpha-insect toxin from the venom of the Chinese scorpion Buthus martensii Karsch.Biochemistry,46(40),11322-11330.
MLA	童孝田,et al."Solution structure of BmK alpha ITO1, an alpha-insect toxin from the venom of the Chinese scorpion Buthus martensii Karsch".Biochemistry 46.40(2007):11322-11330.