Simultaneous Structural Identification of Constituents in Active Herbal Extract of Forsythia Suspensa Using Nuclear Magnetic Resonance/Liquid Chromatography-Mass Spectrometry Parallel Dynamic Spectroscopy
Liu Ying1,2; He Jiu-Ming1,2; Sun Rui-Xiang3; Liu Chao3; Zhang Rui-Ping1,2; Shi Jian-Gong1,2; Zeper, Abliz1,2
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2011-03-01
卷号39期号:3页码:323-329
关键词Nuclear magnetic resonance/liquid chromatography/mass spectrometry parallel dynamic spectroscopy Incomplete separation strategy Simultaneous structural identification in mixture Forsythia Suspensa Phenylethanoid glycosides
ISSN号0253-3820
DOI10.3724/SP.J.1096.2011.00323
英文摘要Based on an incomplete separation strategy, the crude extract of Forsythia Suspensa was separated into a series of fractions with the concentration of constituent dynamic variation by RP preparative chromatography. NMR/LC-MS parallel dynamic spectroscopic (PDS) method was applied to identify the phenylethanoid glycosides in these fractions. The complementary spectral information of the same individual constituent in the crude extract was simultaneously obtained from the spectra of mixtures, including retention time (R(t)), mass/charge (m/z) and chemical shift (delta). Seven constituents were identified successfully, including a new phenylethanoid glycoside and a novel Dimeric-2-(4-aminophenyl) ethyl-beta-D-glucopyranoside which is the first example of dimeric phenylethanol alkaloid glycoside. The results demonstrated that NMR/LC-MS PDS can be used to identify the constituents with more complicated structures from herbs and their active extracts. Especially, this method is suitable for analysis and identification of minimum-quantity constituents and constituents with similar polarity which are hardly obtained in routine experimental conditions. In addition, this method has the advantage of rapid discovering and identifying new compounds from complex mixtures, which can greatly improve the analytical efficiency.
WOS研究方向Chemistry
语种英语
出版者ELSEVIER SCIENCE INC
WOS记录号WOS:000290841800006
内容类型期刊论文
源URL[http://119.78.100.204/handle/2XEOYT63/12912]  
专题中国科学院计算技术研究所期刊论文_英文
通讯作者Zeper, Abliz
作者单位1.Chinese Acad Med Sci, Inst Mat Med, Key Lab Bioact Subst & Resource Utilizat Chinese, Minist Educ, Beijing 100050, Peoples R China
2.Peking Union Med Coll, Beijing 100050, Peoples R China
3.Chinese Acad Sci, Inst Comp Technol, Beijing 100080, Peoples R China
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GB/T 7714
Liu Ying,He Jiu-Ming,Sun Rui-Xiang,et al. Simultaneous Structural Identification of Constituents in Active Herbal Extract of Forsythia Suspensa Using Nuclear Magnetic Resonance/Liquid Chromatography-Mass Spectrometry Parallel Dynamic Spectroscopy[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2011,39(3):323-329.
APA Liu Ying.,He Jiu-Ming.,Sun Rui-Xiang.,Liu Chao.,Zhang Rui-Ping.,...&Zeper, Abliz.(2011).Simultaneous Structural Identification of Constituents in Active Herbal Extract of Forsythia Suspensa Using Nuclear Magnetic Resonance/Liquid Chromatography-Mass Spectrometry Parallel Dynamic Spectroscopy.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,39(3),323-329.
MLA Liu Ying,et al."Simultaneous Structural Identification of Constituents in Active Herbal Extract of Forsythia Suspensa Using Nuclear Magnetic Resonance/Liquid Chromatography-Mass Spectrometry Parallel Dynamic Spectroscopy".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 39.3(2011):323-329.
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