Fluid flow-induced calcium response in early or late differentiated osteoclasts

	Fluid flow-induced calcium response in early or late differentiated osteoclasts
	Li P(李平); Hu M(胡漫); Sun SJ(孙树津); Zhang Y(章燕); Gao YX(高宇欣); Long M(龙勉); Huo B(霍波); Zhang D; Huo, B
刊名	ANNALS OF BIOMEDICAL ENGINEERING
	2012-09-01
通讯作者邮箱	huobo@imech.ac.cn;dingz@china.com.cn
卷号	40 期号:9 页码:1874-1883
关键词	Osteoclasts Fluid shear stress Calcium oscillation Induction time Differentiation Mechanical Strain Rat Osteoclasts Bone-Resorption Activation Expression Cells Ca2+
ISSN号	0090-6964
通讯作者	Huo, B ; Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv, 15 N 4th Ring Rd, Beijing 100190, Peoples R China.
产权排序	[Hu, Man;Sun, Shujin; Zhang, Yan; Gao, Yuxin; Long, Mian; Huo, Bo] Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv, Beijing 100190, Peoples R China; [Li, Ping; Zhang, Ding] Chinese Acad Med Sci, Peking Union Med Coll Hosp, Fac Surg, Dept Stomatol, Beijing 100730, Peoples R China
合作状况	国内
中文摘要	Intracellular calcium oscillation caused by receptor activator of nuclear factor kappa-B ligand has been demonstrated to promote the differentiation of osteoclasts. Osteoclasts are recruited on the surface of trabeculae, and are exposed to fluid flow caused by the deformation of the bone matrix. However, the roles of fluid shear stress (FSS) on calcium response during the differentiation process of osteoclasts are still unknown. In the current study, the formation of tartrate-resistant acid phosphatase-positive, multinucleated osteoclasts from RAW264.7 macrophage cells were induced by co-culturing them with the conditioned medium from MC3T3-E1 osteoblasts. The in situ observations showed a high correlation between the area and the nuclear number of osteoclasts. The cells were stimulated by FSS at different levels (1 or 10 dyne/cm(2)) before (0 day) or after being induced for 4 or 8 days. The mechanically-induced calcium response was recorded and analyzed. The results indicated a different property of calcium oscillation for the osteoclasts in different fusion stages (i.e., more calcium-responsive peaks appeared in small osteoclasts than those in the larger ones). The rates of calcium influx decreased and the time of recovery in osteoclast cytosol increased along with the fusion of osteoclasts. In addition, increasing the FSS level enhanced the calcium oscillation of osteoclasts at early induction (4 days). However, this effect was weakened at the late induction (8 days). The present work could help provide understanding regarding the mechanism of the involvement of calcium in mechanically induced bone remodeling.
学科主题	生物力学
分类号	二类/Q2
收录类别	SCI ; EI
资助信息	This work was supported by the National Natural Science Foundation of China [30970707 (BH) and 31070829 (DZ)], the National Key Basic Research Foundation of China (2011CB710904), and the Knowledge Innovation Project of the CAS (KJCX2-YW-L08) (ML).
原文出处	http://dx.doi.org/10.1007/s10439-012-0554-z
语种	英语
WOS记录号	WOS:000307400900003
公开日期	2013-01-18
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/46584]
专题	力学研究所_国家微重力实验室
通讯作者	Huo, B
推荐引用方式 GB/T 7714	Li P,Hu M,Sun SJ,et al. Fluid flow-induced calcium response in early or late differentiated osteoclasts[J]. ANNALS OF BIOMEDICAL ENGINEERING,2012,40(9):1874-1883.
APA	Li P.,Hu M.,Sun SJ.,Zhang Y.,Gao YX.,...&Huo, B.(2012).Fluid flow-induced calcium response in early or late differentiated osteoclasts.ANNALS OF BIOMEDICAL ENGINEERING,40(9),1874-1883.
MLA	Li P,et al."Fluid flow-induced calcium response in early or late differentiated osteoclasts".ANNALS OF BIOMEDICAL ENGINEERING 40.9(2012):1874-1883.