Calcium response in osteocytic networks under steady and oscillatory fluid flow

	Calcium response in osteocytic networks under steady and oscillatory fluid flow
	Lu XL; Huo B(霍波); Park M; Guo XE
刊名	BONE
	2012-09-01
通讯作者邮箱	ed.guo@columbia.edu
卷号	51 期号:3 页码:466-473
关键词	Osteocyte Mechanotransduction Calcium response Fluid flow Steady Oscillatory Nitric-Oxide Synthase Bone Cell Mechanotransduction Functional Gap-Junctions Osteoblastic Cells Shear-Stress MC3T3-E1 Osteoblasts Molecular Regulation MLO-Y4 Osteocytes Mechanical Strain ATP Release
ISSN号	8756-3282
通讯作者	Guo, XE ; Columbia Univ, Dept Biomed Engn, Bone Bioengn Lab, 351 Engn Terrace,1210 Amsterdam Ave, New York, NY 10027 USA.
产权排序	[Lu, X. Lucas;Huo, Bo; Park, Miri; Guo, X. Edward] Columbia Univ, Dept Biomed Engn, Bone Bioengn Lab, New York, NY 10027 USA; [Lu, X. Lucas; Park, Miri] Univ Delaware, Dept Mech Engn, Newark, DE 19716 USA; [Huo, Bo] Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China
合作状况	国际
中文摘要	The fluid flow in the lacunar-canalicular system of bone is an essential mechanical stimulation on the osteocyte networks. Due to the complexity of human physical activities, the fluid shear stress on osteocyte bodies and processes consists of both steady and oscillatory components. In this study, we investigated and compared the intracellular calcium ([Ca2+](i)) responses of osteocytic networks under steady and oscillatory fluid flows. An in vitro osteocytic network was built with MLO-Y4 osteocyte-like cells using micro-patterning techniques to simulate the in vivo orderly organization of osteocyte networks. Sinusoidal oscillating fluid flow or unidirectional steady flow was applied on the cell surface with 2 Pa peak shear stress. It was found that the osteocytic networks were significantly more responsive to steady flow than to oscillatory flow. The osteocytes can release more calcium peaks with higher magnitudes at a faster speed under steady flow stimulation. The [Ca2+](i) signaling transients under the steady and oscillatory flows have significantly different spatiotemporal characters, but a similar responsive percentage of cells. Further signaling pathway studies using inhibitors showed that endoplasmic reticulum (ER) calcium store, extracellular calcium source, ATP, PGE(2) and NO related pathways play similar roles in the [Ca2+](i) signaling of osteocytes under either steady or oscillating flow. The spatiotemporal characteristics of [Ca2+](i) transients under oscillating fluid flow are affected more profoundly by pharmacological treatments than under the steady flow. Our findings support the hypothesis that the [Ca2+](i) responses of osteocytic networks are significantly dependent on the profiles of fluid flow.
学科主题	生物力学
分类号	二类
收录类别	SCI
资助信息	NIH grants R21 AR052417, R01 AR052461, and RC1 AR058453 (XEG).
原文出处	http://dx.doi.org/10.1016/j.bone.2012.05.021
语种	英语
WOS记录号	WOS:000307617400019
公开日期	2013-01-18
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/46578]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Lu XL,Huo B,Park M,et al. Calcium response in osteocytic networks under steady and oscillatory fluid flow[J]. BONE,2012,51(3):466-473.
APA	Lu XL,霍波,Park M,&Guo XE.(2012).Calcium response in osteocytic networks under steady and oscillatory fluid flow.BONE,51(3),466-473.
MLA	Lu XL,et al."Calcium response in osteocytic networks under steady and oscillatory fluid flow".BONE 51.3(2012):466-473.