Spreading of human neutrophils on an ICAM-1-immobilized substrate under shear flow

	Spreading of human neutrophils on an ICAM-1-immobilized substrate under shear flow
	Zhan DY(展冬颖); Zhang Y(章燕); Long M(龙勉)
刊名	CHINESE SCIENCE BULLETIN
	2012-03-01
通讯作者邮箱	mlong@imech.ac.cn
卷号	57 期号:7 页码:769-775
关键词	neutrophil spreading beta 2 integrin ICAM-1 shear stress Cell-Surface Adhesion L-Selectin Polymorphonuclear Leukocytes Endothelial-Cells Rolling Adhesions P-Selectin Integrin Affinity Stress ICAM-1
ISSN号	1001-6538
通讯作者	Long, MA ; Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China.
产权排序	[Zhan Dongyan; Zhang Yan; Long Mian] Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China;Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China
中文摘要	Neutrophil (PMN) spreading on endothelium, mediated by the interactions between surface-bound beta 2 integrin and intercellular adhesion molecule-1 (ICAM-1) in the inflammatory cascade, is crucial for PMN post-adhesion and trans-migration in blood flow. The underlying mechanisms by which shear flow regulates PMN spreading dynamics are not well understood. Here, a parallel-plate flow chamber assay was applied to quantify the time course of PMN adhesion and spreading on an ICAM-1-immobilized substrate. Two types of shear flow, steady flows at shear stresses of 0.2, 0.5, and 1 dyne/cm(2) and stepwise flows at 0, 1, and 10 dyne/cm(2), were used to elucidate the impact of shear flow on cell adhesion and spreading. The number of adhered PMNs, the fraction of spreading PMNs and the projected area of spread PMNs were determined and were found to correlate with the distribution of surface-bound beta 2 integrin subunit (CD11a, CD11b, or CD18). The results indicate that PMN spreading on an ICAM-1 substrate is bi-directionally regulated under shear flow. CD11a, CD11b and CD18 subunits of beta 2 integrin contribute distinctly to PMN spreading on ICAM-1 substrates. This work provides new insights into understanding PMN spreading on the endothelium, mediated by beta 2 integrin and ICAM-1 under shear flow.
学科主题	生物力学
分类号	二类
收录类别	SCI
资助信息	The authors are grateful to Dr. Quhuan Li of the South China University of Technology for technical assistance and helpful discussions. This work was supported by the National Natural Science Foundation of China (30730032 and 10902117), Chinese Academy of Sciences Knowledge Innovation Project (KJCX2-YW-L08 and Y2010030), and the National Basic Research Program of China (2011CB710904).
原文出处	http://dx.doi.org/10.1007/s11434-011-4939-4
语种	英语
WOS记录号	WOS:000300771200011
公开日期	2013-01-18
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/46561]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Zhan DY,Zhang Y,Long M. Spreading of human neutrophils on an ICAM-1-immobilized substrate under shear flow[J]. CHINESE SCIENCE BULLETIN,2012,57(7):769-775.
APA	展冬颖,章燕,&龙勉.(2012).Spreading of human neutrophils on an ICAM-1-immobilized substrate under shear flow.CHINESE SCIENCE BULLETIN,57(7),769-775.
MLA	展冬颖,et al."Spreading of human neutrophils on an ICAM-1-immobilized substrate under shear flow".CHINESE SCIENCE BULLETIN 57.7(2012):769-775.