Osteocytic network is more responsive in calcium signaling than osteoblastic network under fluid flow

	Osteocytic network is more responsive in calcium signaling than osteoblastic network under fluid flow
	Lu XL; Huo B(霍波); Chiang V; Guo XE
刊名	JOURNAL OF BONE AND MINERAL RESEARCH
	2012-03-01
通讯作者邮箱	ed.guo@columbia.edu
卷号	27 期号:3 页码:563-574
关键词	OSTEOCYTES OSTEOBLASTS CELL NETWORK MECHANOTRANSDUCTION FLUID FLOW CALCIUM SIGNALING Functional Gap-Junctions Bone Cell Networks MC3T3-E1 Osteoblasts Nitric-Oxide Shear-Stress Prostaglandin Release MLO-Y4 Osteocytes Ca2+ Channels ATP Release Long Bones
ISSN号	0884-0431
通讯作者	Guo, XE ; Columbia Univ, Dept Biomed Engn, Bone Bioenn Lab, 351 Engn Terrace,Mail Code 8904,1210 Amsterdam Av, New York, NY 10027 USA.
产权排序	[Lu, X. Lucas;Huo, Bo; Chiang, Victor; Guo, X. Edward] Columbia Univ, Dept Biomed Engn, Bone Bioenn Lab, New York, NY 10027 USA; [Lu, X. Lucas] Univ Delaware, Dept Mech Engn, Newark, DE 19716 USA; [Huo, Bo] Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China
合作状况	国际
中文摘要	Osteocytes, regarded as the mechanical sensor in bone, respond to mechanical stimulation by activating biochemical pathways and mediating the cellular activities of other bone cells. Little is known about how osteocytic networks respond to physiological mechanical stimuli. In this study, we compared the mechanical sensitivity of osteocytic and osteoblastic networks under physiological-related fluid shear stress (0.5 to 4?Pa). The intracellular calcium ([Ca2+]i) responses in micropatterned in vitro osteoblastic or osteocytic networks were recorded and analyzed. Osteocytes in the network showed highly repetitive spikelike [Ca2+]i peaks under fluid flow stimulation, which are dramatically different from those in the osteoblastic network. The number of responsive osteocytes in the network remained at a constant high percentage (>95%) regardless of the magnitude of shear stress, whereas the number of responsive osteoblasts in the network significantly depends on the strength of fluid flow. All spatiotemporal parameters of calcium signaling demonstrated that osteocytic networks are more sensitive and dynamic than osteoblastic networks, especially under low-level mechanical stimulations. Furthermore, pathway studies were performed to identify the molecular mechanisms responsible for the differences in [Ca2+]i signaling between osteoblastic and osteocytic networks. The results suggested that the T-type voltage-gated calcium channels (VGCC) expressed on osteocytes may play an essential role in the unique kinetics of [Ca2+]i signaling in osteocytic networks, whereas the L-type VGCC is critical for both types of cells to release multiple [Ca2+]i peaks. The extracellular calcium source and intracellular calcium store in ER-, ATP-, PGE2-, NO-, and caffeine-related pathways are found to play similar roles in the [Ca2+]i signaling for both osteoblasts and osteocytes. The findings in this study proved that osteocytic networks possess unique characteristics in sensing and processing mechanical signals.
学科主题	生物力学
分类号	二类/Q1
收录类别	SCI
资助信息	This work was supported by National Institutes of Health (NIH) grants R21 AR052417, R01 AR052461, and RC1 AR058453 (XEG). We acknowledge the contribution of Dr. PuiLeng Isabel Leong for her help in PLC inhibition experiments. We thank Dr. L Bonewald for her generous gift of MLO-Y4 cells.
原文出处	http://dx.doi.org/10.1002/jbmr.1474
语种	英语
WOS记录号	WOS:000300682500007
公开日期	2013-01-18
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/46560]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Lu XL,Huo B,Chiang V,et al. Osteocytic network is more responsive in calcium signaling than osteoblastic network under fluid flow[J]. JOURNAL OF BONE AND MINERAL RESEARCH,2012,27(3):563-574.
APA	Lu XL,Huo B,Chiang V,&Guo XE.(2012).Osteocytic network is more responsive in calcium signaling than osteoblastic network under fluid flow.JOURNAL OF BONE AND MINERAL RESEARCH,27(3),563-574.
MLA	Lu XL,et al."Osteocytic network is more responsive in calcium signaling than osteoblastic network under fluid flow".JOURNAL OF BONE AND MINERAL RESEARCH 27.3(2012):563-574.