Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns

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	Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns
	Lei, Xiao-Ying; Ou, Tong; Zhang, Qi-Ya
刊名	PLOS ONE
	2012-08-13
卷号	7 期号:8 页码:e43033
关键词	MAJOR CAPSID PROTEIN FISH CELL-LINE FAMILY IRIDOVIRIDAE SEQUENCE-ANALYSIS GENE EXPRESSION DNA MORPHOGENESIS REPLICATION TRANSPORT
ISSN号	1932-6203
通讯作者	Lei, XY (reprint author), Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
中文摘要	Background: The complete genome of Rana grylio virus (RGV) was sequenced and analyzed recently, which revealed that RGV 50L had homologues in many iridoviruses with different identities; however, the characteristics and functions of 50L have not been studied yet. Methodology/Principal Findings: We cloned and characterized RGV50L, and revealed 50L functions in virus assembly and gene regulation. 50L encoded a 499-amino acid structural protein of about 85 kDa in molecular weight and contained a nuclear localization signal (NLS) and a helix-extension-helix motif. Drug inhibition assay demonstrated that 50L was an immediate-early (IE) gene. Immuno-fluorescence assay revealed that 50L appeared early and persisted in RGV-infected cells following two distribution patterns. One pattern was that 50L exhibited a cytoplasm-nucleus-viromatrix distribution pattern, and mutagenesis of the NLS motif revealed that localization of 50L in the nucleus was NLS-dependent; the other was that 50L co-localized with viral matrix which plays important roles in virus assembly and the life circle of viruses. Conclusion/Significance: RGV 50L is a novel iridovirus IE gene encoded structural protein which plays important roles in virus assembly.
英文摘要	Background: The complete genome of Rana grylio virus (RGV) was sequenced and analyzed recently, which revealed that RGV 50L had homologues in many iridoviruses with different identities; however, the characteristics and functions of 50L have not been studied yet.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	MAJOR CAPSID PROTEIN ; FISH CELL-LINE ; FAMILY IRIDOVIRIDAE ; SEQUENCE-ANALYSIS ; GENE ; EXPRESSION ; DNA ; MORPHOGENESIS ; REPLICATION ; TRANSPORT
收录类别	SCI
资助信息	National Major Basic Research Program [2010CB126303, 2009CB118704]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Key Technologies R & D Program of China [2012BAD25B00]; National Natural Science Foundation of China [31072239]; FEBL [Y15B121F01]
语种	英语
WOS记录号	WOS:000307500100055
公开日期	2013-01-09
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/17232]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Lei, Xiao-Ying,Ou, Tong,Zhang, Qi-Ya. Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns[J]. PLOS ONE,2012,7(8):e43033.
APA	Lei, Xiao-Ying,Ou, Tong,&Zhang, Qi-Ya.(2012).Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns.PLOS ONE,7(8),e43033.
MLA	Lei, Xiao-Ying,et al."Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns".PLOS ONE 7.8(2012):e43033.