Bioflocculant produced by Chlamydomonas reinhardtii

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	Bioflocculant produced by Chlamydomonas reinhardtii
	Zhu, Chi 1; Chen, Chuanhong 1; Zhao, Liangyuan 1; Zhang, Yihui 1; Yang, Jiaoyan 1; Song, Lirong 2; Yang, Shao 1
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2012-10-01
卷号	24 期号:5 页码:1245-1251
关键词	Bioflocculant Flocculation Flocculating rate Chlamydomonas reinhardtii
ISSN号	0921-8971
通讯作者	Yang, S (reprint author), Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
中文摘要	Bioflocculants of Chlamydomonas reinhardtii were investigated under axenic conditions. C. reinhardtii was found to produce significant amounts of bioflocculants. Flocculating activity by C. reinhardtii began in the linear phase of growth and continued until the end of the stationary phase. The highest flocculating efficiency of the culture broth was 97.06%. The purified C. reinhardtii bioflocculant was composed of 42.1% (w/w) proteins, 48.3% carbohydrates, 8.7% lipids, and 0.01% nucleic acid. The optimum condition for bioflocculant production of C. reinhardtii was as follows: under temperature of 15A degrees C to 25A degrees C, pH 6-10 and illumination of 40-60 mu mol photons m(-2) s(-1). The bioflocculants produced by C. reinhardtii showed maximum activity in pH ranges from 2 to 10. The flocculating activity was significantly enhanced by the addition of CaCl2 as a co-flocculant at an optimal concentration of 4.5 mM.
英文摘要	Bioflocculants of Chlamydomonas reinhardtii were investigated under axenic conditions. C. reinhardtii was found to produce significant amounts of bioflocculants. Flocculating activity by C. reinhardtii began in the linear phase of growth and continued until the end of the stationary phase. The highest flocculating efficiency of the culture broth was 97.06%. The purified C. reinhardtii bioflocculant was composed of 42.1% (w/w) proteins, 48.3% carbohydrates, 8.7% lipids, and 0.01% nucleic acid. The optimum condition for bioflocculant production of C. reinhardtii was as follows: under temperature of 15A degrees C to 25A degrees C, pH 6-10 and illumination of 40-60 mu mol photons m(-2) s(-1). The bioflocculants produced by C. reinhardtii showed maximum activity in pH ranges from 2 to 10. The flocculating activity was significantly enhanced by the addition of CaCl2 as a co-flocculant at an optimal concentration of 4.5 mM.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
关键词[WOS]	MICROBIAL FLOCCULANT ; RHODOCOCCUS-ERYTHROPOLIS ; CYANOBACTERIAL BIOFLOCCULANT ; CORYNEBACTERIUM-GLUTAMICUM ; CULTURE CONDITIONS ; NOCARDIA-AMARAE ; BIOPOLYMER ; MICROORGANISMS ; ACIDS ; PURIFICATION
收录类别	SCI
资助信息	National Major Science and Technology Projects for Water Pollution Control and River Management Project [2008ZX07211-003, 2008ZX07105-004]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FB12]; National Natural Science Foundation of China [30900054]
语种	英语
WOS记录号	WOS:000308423100027
公开日期	2013-01-09
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/17288]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	1.Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China 2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Chi,Chen, Chuanhong,Zhao, Liangyuan,et al. Bioflocculant produced by Chlamydomonas reinhardtii[J]. JOURNAL OF APPLIED PHYCOLOGY,2012,24(5):1245-1251.
APA	Zhu, Chi.,Chen, Chuanhong.,Zhao, Liangyuan.,Zhang, Yihui.,Yang, Jiaoyan.,...&Yang, Shao.(2012).Bioflocculant produced by Chlamydomonas reinhardtii.JOURNAL OF APPLIED PHYCOLOGY,24(5),1245-1251.
MLA	Zhu, Chi,et al."Bioflocculant produced by Chlamydomonas reinhardtii".JOURNAL OF APPLIED PHYCOLOGY 24.5(2012):1245-1251.