Identification of a novel thylakoid protein gene involved in cold acclimation in cyanobacteria

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	Identification of a novel thylakoid protein gene involved in cold acclimation in cyanobacteria
	Li, Weizhi; Gao, Hong; Yin, Chuntao; Xu, Xudong
刊名	MICROBIOLOGY-SGM
	2012-09-01
卷号	158 期号:Part 9 页码:2440-2449
关键词	SP PCC 6803 SYNECHOCYSTIS-SP PCC-6803 CHILL-LIGHT TOLERANCE SP STRAIN PCC-6803 LOW-TEMPERATURE PHOTOSYSTEM-II SYNECHOCOCCUS SP LIPID DIFFUSION BTPA PROTEIN MEMBRANES
ISSN号	1350-0872
通讯作者	Xu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.
中文摘要	In cyanobacteria, genes involved in cold acclimation can be upregulated in response to cold stress with or without light. By inactivating 17 such genes in Synechocystis sp. PCC 6803, slr0815 (ccr2) was identified to be a novel gene required for survival at 15 degrees C. It was upregulated by cold stress in the light. Upon exposure to low temperature, a ccr2-null mutant showed greatly reduced photosynthetic and respiratory activities within 12 h relative to the wild-type. At 48 h, the photosystem (PS)II-mediated electron transport in the mutant was reduced to less than one-third of the wild-type level, and the duration of electron transfer from the OB binding site of PSII to PSI was increased to about eight times the wild-type level, whereas the PSI-mediated electron transport remained unchanged. Using an antibody against GFP, a Ccr2-GFP fusion protein was localized to the thylakoid membrane rather than the cytoplasmic and outer membranes. Homologues to Ccr2 can be found in most cyanobacteria, algae and higher plants with sequenced genomes. Ccr2 is probably representative of a group of novel thylakoid proteins involved in acclimation to cold or other stresses.
英文摘要	In cyanobacteria, genes involved in cold acclimation can be upregulated in response to cold stress with or without light. By inactivating 17 such genes in Synechocystis sp. PCC 6803, slr0815 (ccr2) was identified to be a novel gene required for survival at 15 degrees C. It was upregulated by cold stress in the light. Upon exposure to low temperature, a ccr2-null mutant showed greatly reduced photosynthetic and respiratory activities within 12 h relative to the wild-type. At 48 h, the photosystem (PS)II-mediated electron transport in the mutant was reduced to less than one-third of the wild-type level, and the duration of electron transfer from the OB binding site of PSII to PSI was increased to about eight times the wild-type level, whereas the PSI-mediated electron transport remained unchanged. Using an antibody against GFP, a Ccr2-GFP fusion protein was localized to the thylakoid membrane rather than the cytoplasmic and outer membranes. Homologues to Ccr2 can be found in most cyanobacteria, algae and higher plants with sequenced genomes. Ccr2 is probably representative of a group of novel thylakoid proteins involved in acclimation to cold or other stresses.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	SP PCC 6803 ; SYNECHOCYSTIS-SP PCC-6803 ; CHILL-LIGHT TOLERANCE ; SP STRAIN PCC-6803 ; LOW-TEMPERATURE ; PHOTOSYSTEM-II ; SYNECHOCOCCUS SP ; LIPID DIFFUSION ; BTPA PROTEIN ; MEMBRANES
收录类别	SCI
资助信息	State Key Basic Research Development Program of China [2008CB418001]; National Natural Science Foundation of China [30825003]
语种	英语
WOS记录号	WOS:000309852800021
公开日期	2013-01-09
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/17276]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Li, Weizhi,Gao, Hong,Yin, Chuntao,et al. Identification of a novel thylakoid protein gene involved in cold acclimation in cyanobacteria[J]. MICROBIOLOGY-SGM,2012,158(Part 9):2440-2449.
APA	Li, Weizhi,Gao, Hong,Yin, Chuntao,&Xu, Xudong.(2012).Identification of a novel thylakoid protein gene involved in cold acclimation in cyanobacteria.MICROBIOLOGY-SGM,158(Part 9),2440-2449.
MLA	Li, Weizhi,et al."Identification of a novel thylakoid protein gene involved in cold acclimation in cyanobacteria".MICROBIOLOGY-SGM 158.Part 9(2012):2440-2449.