A Fully Automated System for Measuring Cellular Mechanical Properties

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	A Fully Automated System for Measuring Cellular Mechanical Properties
	Wang ZB(王智博); Liu LQ(刘连庆); Wang YC(王越超); Xi N(席宁); Dong ZL(董再励); Li M(李密); Yuan S(袁帅)
刊名	Journal of Laboratory Automation
	2012
卷号	17 期号:6 页码:443-448
关键词	automation mechanical properties of cells atomic force microscopy (AFM) local scan
通讯作者	刘连庆
产权排序	1
中文摘要	As a novel effective label-free biomarker, the mechanical properties of cells have become increasingly important. However, the current methods of mapping cellular mechanical properties are mostly carried out manually, resulting in measurements that are time-consuming with low efficiency. In this article, a fully automated system of measuring the mechanical properties of cells based on atomic force microscopy (AFM) is proposed. In this system, the cells are recognized using an image-processing method and the relative position of the cell, and the AFM tip is accurately calibrated by the local scan method, meaning that the mechanical properties of cells can be measured sequentially without performing the step of AFM imaging. In addition, with the implementation of the automation, the high-throughput measurement of cellular mechanical properties can be performed rapidly. The capability of our system is validated on Raji cells, and the results indicate that the measurement rate of our system is 26 times faster than that of the traditional manual method, providing the technology for high-throughput measurement of cellular mechanical properties.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	ATOMIC-FORCE MICROSCOPY ; CELLS ; AFM
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000313344200005
公开日期	2012-12-28
内容类型	期刊论文
源URL	[http://ir.sia.cn/handle/173321/10330]
专题	沈阳自动化研究所_机器人学研究室
推荐引用方式 GB/T 7714	Wang ZB,Liu LQ,Wang YC,et al. A Fully Automated System for Measuring Cellular Mechanical Properties[J]. Journal of Laboratory Automation,2012,17(6):443-448.
APA	Wang ZB.,Liu LQ.,Wang YC.,Xi N.,Dong ZL.,...&Yuan S.(2012).A Fully Automated System for Measuring Cellular Mechanical Properties.Journal of Laboratory Automation,17(6),443-448.
MLA	Wang ZB,et al."A Fully Automated System for Measuring Cellular Mechanical Properties".Journal of Laboratory Automation 17.6(2012):443-448.