莎能奶山羊皮肤成纤维细胞几种冷冻保存方法的研究

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	莎能奶山羊皮肤成纤维细胞几种冷冻保存方法的研究
	王亮; 刘婷婷; 彭涛; 帅志强; 张达江; 朱海; 陈军; 李卫华; 汪汉卿
刊名	西北农业学报
	2005
卷号	14 期号:6 页码:40914
关键词	莎能奶山羊成纤维细胞细胞培养冷冻保存活细胞率
ISSN号	1004-1389
其他题名	analyses research of several cryopreservation methods for skin fibroblasts of shaneng milk goat
中文摘要	以莎能奶山羊皮肤组织为材料进行细胞培养,通过分离纯化建立了莎能奶山羊皮肤成纤维细胞系.纯化培养的成纤维细胞在冷冻保护剂和血清成分相同的条件下选用 5种具有不同预冷平衡方式和预冷时间的方法进行冷冻保存,5个月后通过对成纤维细胞复苏后的活力对比分析,方法3(70%细胞悬液+20%胎牛血清+10 %DMSO;先在4 C预冷平衡0.5 h,接着液氮罐口气态氮悬挂4 h,然后沉入液氮)冻存细胞解冻后胎盘蓝染色细胞活力分析,活细胞率为84.8%,培养48 h后细胞贴壁率为85.4%,明显高于其他几种方法.
英文摘要	Through isolation and pure culture of Shaneng milk goat fibroblasts with skin tissue, fibroblast line was established. On conditions of the same cryoprotectant and FCS concentration Pure Shaneng milk goat fibroblasts cultured were cryopreservated in five cryopreservation methods with different pre-cool and balance ways and pre-cool time, through the contrast and analyses of cell vigor after five months cryopreservation, method 3 (70% cell suspension -f 20%FCS + 10% DMSO; cryopreservation procedure: first fibroblasts were pre-cooled and balanced for half hour at 4 C , and second hanged in gaseous nitrogen in close to the mouth of nitrogen canister for 4 hours, and finally sunk into liquid nitrogen. ) was the best cryopreservation method, it could maintain 84. 8% of living cell rate through cell vigor test after cells were revived and stained by Trypan Blue and 85. 4% of cell adherence rate after cells were revived and cultured for 48 hours, living cell rate and cell adherence rate of method 3 superior to other four methods.
学科主题	Agriculture (provided by Thomson Reuters)
公开日期	2012-11-29
内容类型	期刊论文
源URL	[http://ir.xjipc.cas.cn/handle/365002/2242]
专题	新疆理化技术研究所_资源化学研究室
作者单位	中国科学院兰州化学物理研究所甘肃省天然药物重点实验室；新疆金牛生物股份有限公司；中国科学院新疆理化技术研究所
推荐引用方式 GB/T 7714	王亮,刘婷婷,彭涛,等. 莎能奶山羊皮肤成纤维细胞几种冷冻保存方法的研究[J]. 西北农业学报,2005,14(6):40914.
APA	王亮.,刘婷婷.,彭涛.,帅志强.,张达江.,...&汪汉卿.(2005).莎能奶山羊皮肤成纤维细胞几种冷冻保存方法的研究.西北农业学报,14(6),40914.
MLA	王亮,et al."莎能奶山羊皮肤成纤维细胞几种冷冻保存方法的研究".西北农业学报 14.6(2005):40914.