The rupestonic acid (compound 1) was isolated from the Chinese herbal medicine Artemisiarupestris L. (its Chinese name being Yizhihao). Then, 13 rupestonic acid amide derivatives 2a～2m weresynthesized using rupestonic acid and organic amines in the presence of DCC, HOBt/DMAP and theirstructures were comfirmed by the methods of IR, 1HNMR, and ESI-MS. All compounds were preliminarilyassayed in vitro against influenza A3, B, herpes simplex I (HSV-I) and herpes simplex II (HSV-II) viruses,showing that 2a was inhibitive to both influenza A3 and B viruses, and had the higher inhibition against influenzaB virus than the parent compound 1. The inhibition of 2d against influenza B virus was 16-foldhigher than that of the parent compound and 2e was more active both to HSV-I and HSV-II viruses .