One-step Separation and Purification of Rupestonic Acid and Chrysosptertin B from Artemisia rupestris L. by High-speed Counter-current Chromatography

CORC > 新疆理化技术研究所 > 中国科学院新疆理化技术研究所 > 省部共建新疆特有药用资源利用重点实验室

	One-step Separation and Purification of Rupestonic Acid and Chrysosptertin B from Artemisia rupestris L. by High-speed Counter-current Chromatography
	Yang Yi; Gu Dongyu; Yili Abulimiti; Zhao Yongxin; He Dajun; Aisa Haji Akber
刊名	PHYTOCHEMICAL ANALYSIS
	2010
卷号	21 期号:2 页码:205-209
关键词	high-speed counter-current chromatography Artemisia rupestris L. rupestonic acid chrysosptertin B one-step separation
ISSN号	0958-0344
通讯作者	Aisa, HA
英文摘要	Introduction - Artemisia rupestris L. is a well-known traditional Chinese medicinal plant in Xinjiang. Rupestonic acid is the main active ingredient of A. rupestris L., and has been chosen as a 'marker compound' for the chemical evaluation or quality control of A. rupestris L. and its products. Although HSCCC separation method was developed before, the separation was performed with two steps using the same solvent system, which were time-consuming and waste of the solvents. Objective - To develop a simple HSCCC method for the separation and purification of rupestonic acid in a single run. Methodology - The measurement of partition coefficient (K) was introduced to select the two-phase solvent system. The simple HSCCC method was established according to the selected solvent system for separation and purification of rupestonic acid. The purity of target compound was test by HPLC and the structure was identified by MS,(1)H NMR and (13)C NMR. Results - A total of 72.3 mg of rupestonic acid and 53.5 mg of chrysosptertin B with over 95% purity were yielded from 500 mg extracts of Artemisia rupestris L. in one-step separation. Conclusion - The rupestonic acid was separated in a single run by HSCCC. Copyright (C) 2009 John Wiley & Sons, Ltd.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry
收录类别	SCI
WOS记录号	WOS:000275007400011
公开日期	2012-11-29
内容类型	期刊论文
源URL	[http://ir.xjipc.cas.cn/handle/365002/1869]
专题	新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室
作者单位	Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yang Yi,Gu Dongyu,Yili Abulimiti,et al. One-step Separation and Purification of Rupestonic Acid and Chrysosptertin B from Artemisia rupestris L. by High-speed Counter-current Chromatography[J]. PHYTOCHEMICAL ANALYSIS,2010,21(2):205-209.
APA	Yang Yi,Gu Dongyu,Yili Abulimiti,Zhao Yongxin,He Dajun,&Aisa Haji Akber.(2010).One-step Separation and Purification of Rupestonic Acid and Chrysosptertin B from Artemisia rupestris L. by High-speed Counter-current Chromatography.PHYTOCHEMICAL ANALYSIS,21(2),205-209.
MLA	Yang Yi,et al."One-step Separation and Purification of Rupestonic Acid and Chrysosptertin B from Artemisia rupestris L. by High-speed Counter-current Chromatography".PHYTOCHEMICAL ANALYSIS 21.2(2010):205-209.