studies on the effect of column angle in figure-8 centrifugal counter-current chromatography

CORC > 新疆理化技术研究所 > 中国科学院新疆理化技术研究所 > 省部共建新疆特有药用资源利用重点实验室

	studies on the effect of column angle in figure-8 centrifugal counter-current chromatography
	Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
刊名	Journal of Chromatography A
	2011
卷号	1218 期号:36 页码:6128-6134
关键词	Centrifugal counter-current chromatography Figure-8 column Angle between column axis and centrifugal force Retention of the stationary phase Resolution Dipeptide DNP-amino acid
ISSN号	219673
英文摘要	The performance of the figure-8 column configuration in centrifugal counter-current chromatography was investigated by changing the angle between the column axis (a line through the central post and the peripheral post on which the figure-8 coil is wound) and the centrifugal force. The first series of experiments was performed using a polar two-phase solvent system composed of 1-butanol-acetic acid-water (4:1:5, v/v) to separate two dipeptide samples, Trp-Tyr and Val-Tyr, at a flow rate of 0.05. ml/min at 1000. rpm. When the column angle was changed from 0° (column axis parallel to the centrifugal force) to 45° and 45° to 90° (column axis perpendicular to the centrifugal force), peak resolution (Rs) changed from 1.93 (Sf = 37.8%) to 1.54 (Sf = 30.6%), then to 1.31 (Sf = 40.5%) with the lower mobile phase and from 1.21 (Sf = 38.8%) to 1.10 (Sf = 34.4%), then to 0.99 (Sf = 42.2%) with the upper mobile phase, respectively, where the stationary phase retention, Sf, is given in parentheses. The second series of experiments was similarly performed with a more hydrophobic two-phase solvent system composed of hexane-ethyl acetate-methanol-0.1. M hydrochloric acid (1:1:1:1, v/v) to separate three DNP-amino acids, DNP-glu, DNP-β-ala and DNP-ala, at a flow rate of 0.05. ml/min at 1000. rpm. When the column angle was altered from 0° to 45° and 45° to 90°, Rs changed from 1.77 (1st peak/2nd peak) and 1.52 (2nd peak/3rd peak) (Sf = 27.3%) to 1.24 and 1.02 (Sf = 35.4%), then to 1.69 and 1.49 (Sf = 42.1%) with the lower mobile phase, and from 1.73 and 0.84 (SF = 41.2%) to 1.44 and 0.73 (Sf = 45.6%), then to 1.21 and 0.63 (Sf = 55.6%) with the upper mobile phase, respectively. The performance of figure-8 column at 0° and 90° was also compared at different flow rates. The results show that Rs was increased with decreased flow rate yielding the highest value at the 0° column angle with lower mobile phase. The overall results of our studies indicated that a 0° column angle for the figure-8 column enhances the mixing of two phases in the column to improve peak resolution while decreasing the stationary phase retention by interrupting the laminar flow of the mobile phase.
收录类别	SCI
WOS记录号	WOS:000294523200018
公开日期	2012-11-29
内容类型	期刊论文
源URL	[http://ir.xjipc.cas.cn/handle/365002/1458]
专题	新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室
作者单位	NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Chem Plant Resources Arid Reg, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. studies on the effect of column angle in figure-8 centrifugal counter-current chromatography[J]. Journal of Chromatography A,2011,1218(36):6128-6134.
APA	Yang Yi,Gu Dongyu,Aisa Haji Akber,&Ito Yoichiro.(2011).studies on the effect of column angle in figure-8 centrifugal counter-current chromatography.Journal of Chromatography A,1218(36),6128-6134.
MLA	Yang Yi,et al."studies on the effect of column angle in figure-8 centrifugal counter-current chromatography".Journal of Chromatography A 1218.36(2011):6128-6134.