Salinity-induced changes in protein expression in the halophytic plant Nitraria sphaerocarpa

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国科学院青藏高原研究所昆明部

	Salinity-induced changes in protein expression in the halophytic plant Nitraria sphaerocarpa
	Chen, Jinhui 1; Cheng, Tielong 2; Wang, Pengkai 1; Liu, Weidong 1; Xiao, Jiao 1; Yang, Yunqiang4 ; Hu, Xiangyang 4; Jiang, Zeping 3; Zhang, Shougong 3; Shi, Jisen 1
刊名	JOURNAL OF PROTEOMICS
	2012-09-18
卷号	75 期号:17 页码:5226-5243
关键词	Comparative proteomics Salinity stress Nitraria sphaerocarpa Cell suspension
ISSN号	1874-3919
通讯作者	Zhang, SG (reprint author), 1 DongXiao Fu, Beijing 100091, Peoples R China.,shougong.zhang@caf.ac.cn ; jshi@njfu.edu.cn
英文摘要	Salinity is a major abiotic stress that inhibits plant growth and development. Plants have evolved complex adaptive mechanisms that respond to salinity stress. However, an understanding of how plants respond to salinity stress is far from being complete. In particular, how plants survive salinity stress via alterations to their intercellular metabolic networks and defense systems is largely unknown. To delineate the responses of Nitraria sphaerocarpa cell suspensions to salinity, changes in their protein expression patterns were characterized by a comparative proteomic approach. Cells that had been treated with 150mM NaCl for 1, 3, 5, 7, or 9days developed several stress-related phenotypes, including those affecting morphology and biochemical activities. Of similar to 1100 proteins detected in 2-DE gel patterns, 130 proteins showed differences in abundance with more than 1.5-fold when cells were stressed by salinity. All but one of these proteins was identified by MS and database searching. The 129 spots contained 111 different proteins, including those involved in signal transduction, cell rescue/defense, cytoskeleton and cell cycle, protein folding and assembly, which were the most significantly affected. Taken together, our results provide a foundation to understand the mechanism of salinity response. (C) 2012 Elsevier B.V. All rights reserved.
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	STRESS SIGNAL-TRANSDUCTION ; HETEROTRIMERIC G-PROTEINS ; WALL-ASSOCIATED KINASES ; LENGTH CDNA MICROARRAY ; ARABIDOPSIS-THALIANA ; OSMOTIC-STRESS ; SALT STRESS ; RECEPTOR KINASE ; OXIDATIVE STRESS ; CELL EXPANSION
收录类别	SCI
资助信息	National Science Foundation of China [30901156, 31170619, 30930077]; Chinese National Forest Bureau ('948') [2009-4-24, 201004049]; Natural Science Foundation of Jiangsu University [09KJA220001]; National Nonprofit Institute Research Grant of CAF [CAFYBB2011001];
语种	英语
WOS记录号	WOS:000309493400008
公开日期	2012-11-30
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/15790]
专题	昆明植物研究所_中国科学院青藏高原研究所昆明部
作者单位	1.Nanjing Forestry Univ, Key Lab Forest Genet & Biotechnol, Minist Educ, Nanjing 210037, Jiangsu, Peoples R China 2.Chinese Acad Forestry, Div Res Management, Beijing 100091, Peoples R China 3.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China 4.Chinese Acad Sci, Inst Tibet Plateau Res Kunming, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Peoples R China
推荐引用方式 GB/T 7714	Chen, Jinhui,Cheng, Tielong,Wang, Pengkai,et al. Salinity-induced changes in protein expression in the halophytic plant Nitraria sphaerocarpa[J]. JOURNAL OF PROTEOMICS,2012,75(17):5226-5243.
APA	Chen, Jinhui.,Cheng, Tielong.,Wang, Pengkai.,Liu, Weidong.,Xiao, Jiao.,...&Shi, Jisen.(2012).Salinity-induced changes in protein expression in the halophytic plant Nitraria sphaerocarpa.JOURNAL OF PROTEOMICS,75(17),5226-5243.
MLA	Chen, Jinhui,et al."Salinity-induced changes in protein expression in the halophytic plant Nitraria sphaerocarpa".JOURNAL OF PROTEOMICS 75.17(2012):5226-5243.