Self-assembly of carboxymethyl konjac glucomannan-g-poly(ethylene glycol) and (alpha-cyclodextrin) to biocompatible hollow nanospheres for glucose oxidase encapsulation

	Self-assembly of carboxymethyl konjac glucomannan-g-poly(ethylene glycol) and (alpha-cyclodextrin) to biocompatible hollow nanospheres for glucose oxidase encapsulation
	Li, Quan ; Xia, Bing ; Branham, Mickey ; Ha, Wei ; Wu, Hao ; Peng, Shu-Lin ; Ding, Li-Sheng ; Li, Bang-Jing ; Zhang, Sheng
刊名	CARBOHYDRATE POLYMERS
	2011
卷号	86 期号:1 页码:120-126
关键词	Biocompatibility Carboxymethyl konjac glucomannan Enzyme encapsulation Nanospheres Self-assembly
ISSN号	0144-8617
通讯作者	Li, Bang-Jing ; Zhang, Sheng
产权排序	1
英文摘要	The self-assembly of rod-coil carboxymethyl konjac glucomannan-graft-poly(ethylene glycol) (CKGM-g-PEG) and alpha-cyclodextrin (alpha-CD) complexes were investigated and used as encapsulating hollow nanospheres for the enzyme glucose oxidase (GOX) in aqueous solution. These hollow nanospheres exhibited "cell-like" semi-permeability allowing enzyme substrates to pass through the surface while restricting the encapsulated enzyme (i.e. GOX) to the interior. Encapsulated GOX exhibited higher thermostability, optimal enzymatic activity over a wider pH range and improved storage stability in comparison to free un-encapsulated GOX. In addition, these CKGM-g-PEG/alpha-CD hollow nanospheres showed in vitro biocompatibility when exposed to L929 cells when tested using MIT viability assay. These studies suggested that self-assembly of CKGM-g-PEG and alpha-CD to form stable nanospheres may be an effective method for enzyme encapsulation with numerous biomedical applications. (C) 2011 Elsevier Ltd. All rights reserved.
学科主题	Chemistry; Polymer Science
收录类别	SCI
资助信息	National Natural Science Foundation of China (NSFC)[21074138, 51073107, 50703025]; State Key Laboratory of Polymer Materials Engineering (Sichuan University)[KF201003]
语种	英语
WOS记录号	WOS:000293114900015
公开日期	2012-11-09
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/23536]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Li, Quan,Xia, Bing,Branham, Mickey,et al. Self-assembly of carboxymethyl konjac glucomannan-g-poly(ethylene glycol) and (alpha-cyclodextrin) to biocompatible hollow nanospheres for glucose oxidase encapsulation[J]. CARBOHYDRATE POLYMERS,2011,86(1):120-126.
APA	Li, Quan.,Xia, Bing.,Branham, Mickey.,Ha, Wei.,Wu, Hao.,...&Zhang, Sheng.(2011).Self-assembly of carboxymethyl konjac glucomannan-g-poly(ethylene glycol) and (alpha-cyclodextrin) to biocompatible hollow nanospheres for glucose oxidase encapsulation.CARBOHYDRATE POLYMERS,86(1),120-126.
MLA	Li, Quan,et al."Self-assembly of carboxymethyl konjac glucomannan-g-poly(ethylene glycol) and (alpha-cyclodextrin) to biocompatible hollow nanospheres for glucose oxidase encapsulation".CARBOHYDRATE POLYMERS 86.1(2011):120-126.