Determination of gallic acid and Salidroside in Rhodiola and its preparation by capillary electrophoresis

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Determination of gallic acid and Salidroside in Rhodiola and its preparation by capillary electrophoresis
	Shi YP(师彦平)
刊名	Journal of Analytical Chemistry
	2006
卷号	61 期号:4 页码:365-368
ISSN号	1061-9348
中文摘要	A new, simple, and rapid capillary electrophoresis (CE) method employing hexadimethrine bromide (HDB) as electroosmotic flow (EOF) modifier was developed for the identification and quantitative determination of two pharmaceutically active constituents—gallic acid (GA) and salidroside (S)—in extracts of Rhodiola root and its medicinal preparation. The optimum separation was achieved at pH 11.00 with the use of 10 mM borate buffer containing 0.001% (w/v) of HDB. The applied voltage was ~15 kV and the capillary temperature was kept constant at 25℃. m-Phthalic acid was used as an internal standard for quantification. The calibration dependences exhibited good linearity for the ratios of the concentrations of standard samples and internal standard and the ratios of the peak area of samples and internal standard over the concentration range from 24 to 1200 μg/mL for GA and 2.4 to 72 μg/mL for S. The correlation coefficients were 0.9999 and 0.9997, and the detection limits of the CE method corresponding to a signal-to-noise ratio of three were 6 and 2 μg/mL for GA and S, respectively. The relative standard deviations of the relative migration time and the relative peak area of samples were 0.5 and 4.0% for GA and 1.9 and 5.3% for S. The effects of buffer pH and the concentration of HDB on the resolution were studied systematically. The contents of these two active compounds in Rhodiola root and its preparation were successfully determined over 6 min with satisfactory repeatability and recovery.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the Foundation for Bairen Jihua of CAS in 2000;the National Natural Science Foundation of China (NSFC no. 20475057)
语种	英语
公开日期	2012-10-14
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/1449]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
推荐引用方式 GB/T 7714	Shi YP. Determination of gallic acid and Salidroside in Rhodiola and its preparation by capillary electrophoresis[J]. Journal of Analytical Chemistry,2006,61(4):365-368.
APA	师彦平.(2006).Determination of gallic acid and Salidroside in Rhodiola and its preparation by capillary electrophoresis.Journal of Analytical Chemistry,61(4),365-368.
MLA	师彦平."Determination of gallic acid and Salidroside in Rhodiola and its preparation by capillary electrophoresis".Journal of Analytical Chemistry 61.4(2006):365-368.