Chiral high-performance liquid chromatographic separation of the enantiomers of tetrahydropalmatine and tetrahydroberberine, isolated from Corydalis yanhusuo

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Chiral high-performance liquid chromatographic separation of the enantiomers of tetrahydropalmatine and tetrahydroberberine, isolated from Corydalis yanhusuo
	Shi YP(师彦平); Shi YP(师彦平)
刊名	Anal Bioanal Chem
	2006
卷号	384 页码:939-945
关键词	Enantiomer separation Polysaccharide carbamate phase Specific rotation Circular dichroism Tetrahydropalmatine Tetrahydroberberine
ISSN号	1618-2642
通讯作者	师彦平
中文摘要	HPLC methods have been developed for chiral resolution of the enantiomers of dl-tetrahydropalmatine (THP) and dl-tetrahydroberberine (THB), two active constituents of Corydalis yanhusuo W.T. Wang. On the analytical scale, good baseline separation of the enantiomers was achieved using cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in both normal-phase and polar organic modes. Validation of the analytical methods, including linearity, limits of detection, recovery, and precision, and semipreparative resolution of dl-THP and dl-THB, were achieved with methanol as mobile phase, without any basic additives, in polar organic mode using cellulose tris (3,5-dimethylphenylcarbamate) chiral stationary phases. On the semipreparative scale, small quantities of the individual enantiomers of THP and THB were isolated for study of the chiroptical properties of the individual enantiomers.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the “Bairen Jihua” Foundation of Chinese Academy of Sciences (CAS) in 2000;the National Natural Science Foundation of China (NSFC No.20475057)
语种	英语
WOS记录号	WOS:000236031500012
公开日期	2012-10-14
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/1448]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Shi YP(师彦平); Shi YP(师彦平)
推荐引用方式 GB/T 7714	Shi YP,Shi YP. Chiral high-performance liquid chromatographic separation of the enantiomers of tetrahydropalmatine and tetrahydroberberine, isolated from Corydalis yanhusuo[J]. Anal Bioanal Chem,2006,384:939-945.
APA	师彦平,&师彦平.(2006).Chiral high-performance liquid chromatographic separation of the enantiomers of tetrahydropalmatine and tetrahydroberberine, isolated from Corydalis yanhusuo.Anal Bioanal Chem,384,939-945.
MLA	师彦平,et al."Chiral high-performance liquid chromatographic separation of the enantiomers of tetrahydropalmatine and tetrahydroberberine, isolated from Corydalis yanhusuo".Anal Bioanal Chem 384(2006):939-945.