Simultaneous Determination of Tetrahydropalmatine, Magnolol, Emodin and Chrysophanol in Chinese Herbal Preparation by RP-HPLC-PDA

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Simultaneous Determination of Tetrahydropalmatine, Magnolol, Emodin and Chrysophanol in Chinese Herbal Preparation by RP-HPLC-PDA
	Shi YP(师彦平); Shi YP(师彦平)
刊名	Chromatographia
	2007
卷号	65 期号:9/10 页码:601-606
关键词	Column liquid chromatography RP-HPLC-PDA Dan’an mixture Tetrahydropalmatine Magnolol Emodin and Chrysophanol
ISSN号	0009-5893
通讯作者	师彦平
中文摘要	A reversed phase high performance liquid chromatography coupled with photo-diode array (RP-HPLC-PDA) detection method was proposed for simultaneous determination of tetrahydropalmatine, magnolol, emodin and chrysophanol in a Chinese herbal preparation (Dan’an mixture). The separation was performed on a DiamonsilTM C18 column (250 · 4.6 mm, 5 lm) with methanol and 0.1% phosphoric acid (88:12, v/v) as the mobile phase at the flow-rate of 0.8 mL min)1. Two detection wavelengths were utilized for the quantitative analysis (209 nm for tetrahydropalmatine and magnolol, and 220 nm for emodin and chrysophanol, respectively). A good linear regression relationship (r ‡ 0.9996) between peak-areas and concentrations was obtained over the range of 0.25–50 lg mL)1 for all the analytes. The spike recoveries, measured at three concentration levels, varied from 90.13 to 102.11%. The method was successfully applied to determine the contents of the four compounds in Dan’an mixture.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the National Natural Science Foundation of China (NSFC 20021001);the Tackle Key Projects of Gansu, China (2GS042-A43-014-12)
语种	英语
公开日期	2012-10-11
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/1418]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Shi YP(师彦平); Shi YP(师彦平)
推荐引用方式 GB/T 7714	Shi YP,Shi YP. Simultaneous Determination of Tetrahydropalmatine, Magnolol, Emodin and Chrysophanol in Chinese Herbal Preparation by RP-HPLC-PDA[J]. Chromatographia,2007,65(9/10):601-606.
APA	师彦平,&师彦平.(2007).Simultaneous Determination of Tetrahydropalmatine, Magnolol, Emodin and Chrysophanol in Chinese Herbal Preparation by RP-HPLC-PDA.Chromatographia,65(9/10),601-606.
MLA	师彦平,et al."Simultaneous Determination of Tetrahydropalmatine, Magnolol, Emodin and Chrysophanol in Chinese Herbal Preparation by RP-HPLC-PDA".Chromatographia 65.9/10(2007):601-606.