Analysis of Three Flavonoids in Oxytropis kansuensis Bunge by RP-LC–DAD Coupled with Weighted Least-Squares Linear Regression

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Analysis of Three Flavonoids in Oxytropis kansuensis Bunge by RP-LC–DAD Coupled with Weighted Least-Squares Linear Regression
	Di DL(邸多隆); Jiang SX(蒋生祥); Liu JX(柳军玺)
刊名	Chromatogr-aphia
	2008
卷号	68 页码:773-779
关键词	Column liquid chromatography Reversed phase Oxytropis kansuensis Bunge Flavonoid Weighted least-squares linear regression
ISSN号	0009-5893
通讯作者	邸多隆
中文摘要	For the first time an RP-LC method with diode-array detection has been developed for simultaneous analysis of three flavonoids [rhamnocitrin-3-O-b-D-galactopyranoside-40-O-b-D-glucospyranoside (RGG), rhamnocitrin-3-O-b-D-galactopyranoside (RG), and 10-methoxymedicarpin (MC)] in a methanol extract of Oxytropis kansuensis Bunge whole plant.Separation was achieved on an ODS column within 18 min. The effect of mobile phase pH on separation of the three flavonoids was investigated. Compared with relative errors obtained by use of least-squares linear regression and logarithmic regression for data processing,weighted least-squares linear regression was more accurate. Response was a linear function of concentration in the ranges 0.0091–3.4, 0.013–4.9, and 0.0085–3.2 mg mL-1 for RGG, RG, and MC, respectively, with correlation coefficients >0.9997. The amounts of the three flavonoids in O. kansuensis Bunge were successfully analyzed with satisfactory repeatability and recovery.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the Key Research Project of the Chinese Academy of Sciences (No. KGCX2-SW-213-08) ; the ‘‘Hundred Talents Program’’ of the Chinese Academy of Sciences.
语种	英语
公开日期	2012-10-11
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/1391]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Di DL(邸多隆)
推荐引用方式 GB/T 7714	Di DL,Jiang SX,Liu JX. Analysis of Three Flavonoids in Oxytropis kansuensis Bunge by RP-LC–DAD Coupled with Weighted Least-Squares Linear Regression[J]. Chromatogr-aphia,2008,68:773-779.
APA	邸多隆,蒋生祥,&柳军玺.(2008).Analysis of Three Flavonoids in Oxytropis kansuensis Bunge by RP-LC–DAD Coupled with Weighted Least-Squares Linear Regression.Chromatogr-aphia,68,773-779.
MLA	邸多隆,et al."Analysis of Three Flavonoids in Oxytropis kansuensis Bunge by RP-LC–DAD Coupled with Weighted Least-Squares Linear Regression".Chromatogr-aphia 68(2008):773-779.