Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs
	Jiang SX(蒋生祥); Jiang SX(蒋生祥); Liu X(刘霞)
刊名	Journal of Ethnopharmacology
	2008
卷号	117 期号:3 页码:420-426
关键词	Chi-Zhi-Huang decoction Phase I drug metabolism Cocktail probe drugs High performance liquid chromatography
ISSN号	0378-8741
通讯作者	蒋生祥
中文摘要	Aim of the study: Chi-Zhi-Huang decoction (PGR) is one of the traditional Chinese medicine (TCM) preparations with unique effect on withdrawing jaundice and has been used to treat icteric patients in China for many years. In this research, we aim at to evaluate the potential activity of PGR in restoring hepatic drug metabolism in a damaged liver. Materials and methods: A cocktail approach with caffeine (10 mg/kg), dapsone (10 mg/kg) and chlorzoxazone (20 mg/kg) respectively as probe drug of cytochrome P450 (CYP) isoform of CYP 1A2, 3A4 and 2E1 was used to evaluate its possible effects on Phase I oxidative metabolism. Pretreated with three dosages of PGR water extract (0.75, 1.5 and 3 g/kg, po) for 5 days, male Wistar rats (220–240 g) were intoxicated by phenylisothiocyanate (PITC, 100 mg/kg, po) 24 h before probes intravenous injection. The pharmacokinetics of the probes in the blood was determined simultaneously by HPLC, and their non-compartmental parameterswere used to evaluate the metabolic difference among the groups. Moreover, the levels of liver enzymes (ALT, AST, ALP) and bilirubins were also measured for insight of liver function. Results: The findings in this study suggest that PGR induces CYP 3A4, does not have much effect on CYP 2E1, and inhibits CYP 1A2 at high dosage.Conclusion: The current pharmacokinetic approach allowed the protective effects of PGR on oxidative drug metabolism in damaged liver to be systemically examined and will certainly help in the explanation of synergistic effect of the composites formula.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the Natural Science Foundation of Gansu Province, China (No. 3YS051-A25-022).
语种	英语
WOS记录号	WOS:000256556100005
公开日期	2012-10-11
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/1387]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Jiang SX(蒋生祥); Jiang SX(蒋生祥)
推荐引用方式 GB/T 7714	Jiang SX,Jiang SX,Liu X. Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs[J]. Journal of Ethnopharmacology,2008,117(3):420-426.
APA	蒋生祥,蒋生祥,&刘霞.(2008).Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs.Journal of Ethnopharmacology,117(3),420-426.
MLA	蒋生祥,et al."Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs".Journal of Ethnopharmacology 117.3(2008):420-426.