Preparative isolation and purification of steviol glycosides from Stevia rebaudiana Bertoni using high-speed counter-current chromatography

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Preparative isolation and purification of steviol glycosides from Stevia rebaudiana Bertoni using high-speed counter-current chromatography
	Di DL(邸多隆); Di DL(邸多隆); Huang XY(黄新异)
刊名	Separation and purification technology
	2010
卷号	71 页码:220-224
关键词	High-speed counter-current chromatography Steviol glycosides Stevioside Rebaudioside A Rebaudioside C
ISSN号	1383-5866
通讯作者	邸多隆
中文摘要	Three steviol glycosides, stevioside, rebaudioside A and rebaudioside C were successfully isolated and purified from the extract of leaves of Stevia rebaudiana Bertoni by high-speed counter-current chromatography (HSCCC). Based on the principle of the partition coefficient values (k) for target compounds and the separation factor (˛) between targets compounds, the two-phase solvent system which contains n-hexane–n-butanol–water at an optimized volume ratio of 1.5:3.5:5 (v/v/v) was selected for the HSCCC separation and the lower phase was employed as the mobile phase in the head to tail elution mode. In a single operation, 200mg of the crude extract yielded pure stevioside (54 mg), rebaudioside A (36 mg), and rebaudioside C (13 mg) with the purities of 98.3%, 98.5% and 97.6%, respectively. The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the structures of the three steviol glycosides were identified by 1H NMR, 13C NMR and DEPT-NMR.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the Natural Sciences Foundation of China (20775083);the “Hundred Talents Program” of the Chinese Academy of Sciences (CAS)
语种	英语
WOS记录号	WOS:000277662000013
公开日期	2012-09-29
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/1226]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Di DL(邸多隆); Di DL(邸多隆)
推荐引用方式 GB/T 7714	Di DL,Di DL,Huang XY. Preparative isolation and purification of steviol glycosides from Stevia rebaudiana Bertoni using high-speed counter-current chromatography[J]. Separation and purification technology,2010,71:220-224.
APA	邸多隆,邸多隆,&黄新异.(2010).Preparative isolation and purification of steviol glycosides from Stevia rebaudiana Bertoni using high-speed counter-current chromatography.Separation and purification technology,71,220-224.
MLA	邸多隆,et al."Preparative isolation and purification of steviol glycosides from Stevia rebaudiana Bertoni using high-speed counter-current chromatography".Separation and purification technology 71(2010):220-224.