Determination of aristolochic acid in urine using hollow fiber liquid-phase microextraction combined with high performance liquid chromatography

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Determination of aristolochic acid in urine using hollow fiber liquid-phase microextraction combined with high performance liquid chromatography
	Chen J(陈娟); Shi YP(师彦平); Shi YP(师彦平); Chen J(陈娟)
刊名	Biomed. Chromatogr.
	2010
卷号	24 页码:1350-1355
关键词	hollow fiber liquid-phase microextraction high-performance liquid chromatography aristolochic acid urine sample
ISSN号	0269-3879
通讯作者	师彦平 ; 陈娟
中文摘要	A simple and efficient hollow fiber liquid-phase microextraction (HF-LPME) technique in conjunction with highperformance liquid chromatography is presented for extraction and quantitative determination of aristolochic acid I in human urine samples. Several parameters influencing the efficiency of HF-LPME were investigated and optimized, including extraction solvent, stirring rate, extraction time, pH of donor phase and acceptor phase. Excellent sample clean-upwas observed and good linearity with coefficient of 0.9999 was obtained in the range of 15.4–960 mg/L. This method provided a 230-fold enrichment factor and good repeatabilitywith relative standard deviations (RSD) lower than 6.0%.The limit of detection value for the analyte in urine samplewas 0.01 mg/L at a signal-to-noise ratio of 3.The extraction recovery fromurine sampleswas 61.8% with an RSD of 9.71%.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	National Key Technology Research and Development Program of China (no. 2007BAI37B05);West Light Program of Chinese Academy of Sciences (2007)
语种	英语
公开日期	2012-09-29
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/1199]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Chen J(陈娟); Shi YP(师彦平); Shi YP(师彦平); Chen J(陈娟)
推荐引用方式 GB/T 7714	Chen J,Shi YP,Shi YP,et al. Determination of aristolochic acid in urine using hollow fiber liquid-phase microextraction combined with high performance liquid chromatography[J]. Biomed. Chromatogr.,2010,24:1350-1355.
APA	陈娟,师彦平,师彦平,&陈娟.(2010).Determination of aristolochic acid in urine using hollow fiber liquid-phase microextraction combined with high performance liquid chromatography.Biomed. Chromatogr.,24,1350-1355.
MLA	陈娟,et al."Determination of aristolochic acid in urine using hollow fiber liquid-phase microextraction combined with high performance liquid chromatography".Biomed. Chromatogr. 24(2010):1350-1355.