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	Production of phloroglucinol by Escherichia coli using a stationary-phase promoter
	Cao, Yujin1,2; Xian, Mo1
刊名	BIOTECHNOLOGY LETTERS
	2011-09-01
卷号	33期号:9页码:1853-1858
关键词	Escherichia coli fic promoter phlD Phloroglucinol Plasmid copy number
中文摘要	Escherichia coli was metabolically engineered using a new host-vector system to produce phloroglucinol. The key biosynthetic gene phlD (encoding a type III polyketide synthase) from Pseudomonas fluorescens was expressed in E. coli using the stationary-phase promoter of the fic gene and a high-copy plasmid. In shake-flasks, the engineered strain produced phloroglucinol up to 0.28 g/l with a productivity of 0.014 g/l h. About 9.2% of the glucose consumed was converted to phloroglucinol after 20 h. Compared with the widely used inducible T7 promoter system, this strain did not require IPTG induction and the final titer of phloroglucinol was 22% higher.
英文摘要	Escherichia coli was metabolically engineered using a new host-vector system to produce phloroglucinol. The key biosynthetic gene phlD (encoding a type III polyketide synthase) from Pseudomonas fluorescens was expressed in E. coli using the stationary-phase promoter of the fic gene and a high-copy plasmid. In shake-flasks, the engineered strain produced phloroglucinol up to 0.28 g/l with a productivity of 0.014 g/l h. About 9.2% of the glucose consumed was converted to phloroglucinol after 20 h. Compared with the widely used inducible T7 promoter system, this strain did not require IPTG induction and the final titer of phloroglucinol was 22% higher.
学科主题	生物基化学品
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	GENES ; 2,4-DIACETYLPHLOROGLUCINOL
收录类别	SCI
语种	英语
WOS记录号	WOS:000293752700022
公开日期	2012-11-07
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1112]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
作者单位	1.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Cao, Yujin,Xian, Mo. Production of phloroglucinol by Escherichia coli using a stationary-phase promoter[J]. BIOTECHNOLOGY LETTERS,2011,33(9):1853-1858.
APA	Cao, Yujin,&Xian, Mo.(2011).Production of phloroglucinol by Escherichia coli using a stationary-phase promoter.BIOTECHNOLOGY LETTERS,33(9),1853-1858.
MLA	Cao, Yujin,et al."Production of phloroglucinol by Escherichia coli using a stationary-phase promoter".BIOTECHNOLOGY LETTERS 33.9(2011):1853-1858.

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