Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy

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	Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy
	Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Dong ZL(董再励); Xiao XB(肖秀斌); Zhang WJ(张伟京)
刊名	物理化学学报
	2012
卷号	28 期号:6 页码:1502-1508
关键词	Atomic force microscopy Lymphoma Elasticity Force curve Youngʹs modulus
ISSN号	1000-6818
其他题名	基于AFM的药物刺激前后淋巴瘤活细胞的形貌及弹性的变化
通讯作者	刘连庆 ; 席宁 ; 张伟京
产权排序	1
中文摘要	原子力显微镜(AFM)的发明为研究单个活细胞的形貌结构及物理特性提供了新的技术手段.然而,由于缺少合适的固定方法,利用AFM对动物悬浮活细胞的形貌进行高分辨率成像还面临着巨大的挑战.本文提出一种基于微柱阵列和静电吸附相结合的动物悬浮细胞固定方法.通过微柱阵列的机械钳制和多聚赖氨酸的静电吸附实现了对单个淋巴瘤B细胞的固定,并在此基础上利用AFM动态观测了不同浓度Rituximab刺激下淋巴瘤B细胞的表面形貌及弹性的变化.经过0.2 mg?mL-1的Rituximab刺激2 h后,细胞表面的褶皱增加,细胞的杨氏模量从196 kPa减小到183 kPa.经过0.5 mg?mL-1的Rituximab刺激2 h后,细胞形貌发生显著变化并出现突起结构,细胞的杨氏模量从234 kPa减小到175 kPa.实验结果表明淋巴瘤细胞形貌和弹性变化的幅度随着Rituximab刺激浓度的增加而增加,加深了对Rituximab作用效果的认识.
英文摘要	Atomic force microscopy(AFM) provides a means for characterizing the surface topography and biophysical properties of individual living cells under near-physiological conditions.However,owing to the lack of adequate cellular immobilization methods,AFM imaging of living,suspended mammalian cells is still a big challenge.In this paper,a method is presented for immobilizing individual living B lymphoma cells that combines mechanical trapping with pillar arrays and electrostatic adsorption with poly-L-lysine.In this way,the topography and elasticity changes of individual B lymphoma cells that were stimulated with different concentrations of Rituximab were observed and measured dynamically.When the cell is stimulated by 0.2 mg·mL-1Rituximab for 2 h,the cell topography becomes more corrugated and Young smodulus decreases from 196 to 183 kPa.When the cell is stimulated by 0.5 mg·mL-1Rituximab for 2 h,thecell topography changes more significantly and some tubercles appear,and Young s modulus decreasesfrom 234 to 175 kPa.These results thus provide a unique insight into the effects of Rituximab on individualcells.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Physical
研究领域[WOS]	Chemistry
关键词[WOS]	FIBROBLASTS ; INHIBITION
收录类别	SCI ; CSCD
资助信息	The project was supported by the National Natural Science Foundation of China(60904095,61175103); National High Technology Research and Development Program of China(863)(2009AA03Z316); Chinese Academy of Sciences Foreign Experts Affairs International Partnership Program for Creative Research Teams
语种	英语
WOS记录号	WOS:000305035700031
公开日期	2012-10-24
内容类型	期刊论文
源URL	[http://ir.sia.cn/handle/173321/9989]
专题	沈阳自动化研究所_机器人学研究室
推荐引用方式 GB/T 7714	Li M,Liu LQ,Xi N,et al. Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy[J]. 物理化学学报,2012,28(6):1502-1508.
APA	Li M.,Liu LQ.,Xi N.,Wang YC.,Dong ZL.,...&Zhang WJ.(2012).Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy.物理化学学报,28(6),1502-1508.
MLA	Li M,et al."Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy".物理化学学报 28.6(2012):1502-1508.