Gene mapping of a new coat mutation in mouse

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	Gene mapping of a new coat mutation in mouse
	Li, SR ; Wang, DP ; Lan, H ; Zhang, WY ; Ge, BS ; Li, JQ ; Wang, C ; Lu, YF ; Shi, YY ; Li, RF
刊名	CHINESE SCIENCE BULLETIN
	1999
卷号	44 期号:13 页码:1207-1210
ISSN号	1001-6538
中文摘要	Gene mapping of a mouse coat mutation has been investigated. First, 100 10-bp random primers were used to amplify DNA, but the mutation could not be located by this method because there were no correlation between the amplified products and coat phenotypes. Second, by using Idh1, Car2, Mup1, Pgb1, Hbb, Es10, Es1, Mod1, Gdc1, Ce2, Es3 as genetic markers, linkage test crosses (two-point test) consisting of intercrossing uncovered BALB/c mice (homozygotes) to CBA/N and C57BL/6 mice with normal hair and backcrossing the heterozygotes of the F1 to the uncovered BALB/c mice were made. It was soon evident that the mutation was linked to Es3 on chromosome 11. Furthermore, three-point test was made by using Es3 and D11Mit8 (a microsatellite DNA) as genetic markers. The result showed that the mutation was linked to Es3 with the percentage recombination of (7.89 +/- 2.19)%, and linked to D11Mit8 with the percentage recombination of (26.38 +/- 3.57)%. The percentage recombination between Es3 and D11Mit8 was (32.90 +/- 3.81)%. The mutation was named Uncovered, with the symbol Uncv. According to the recombinations, the loci order was D11Mit8-26.30 +/- 3.57- Uncv-7.89 +/- 2.19-Es3. From the location on the chromosome, it was concluded that the mutation was a new mutation which affected the skin and hair structure of mouse. The Uncv has entered MGD (Mouse Genome Database).
学科主题	Multidisciplinary Sciences
收录类别	SCI-E
原文出处	1999441207.pdf
公开日期	2010-08-24 ; 2010-10-13
内容类型	期刊论文
源URL	[http://159.226.149.42:8080/handle/152453/5161]
专题	昆明动物研究所_其他
推荐引用方式 GB/T 7714	Li, SR,Wang, DP,Lan, H,et al. Gene mapping of a new coat mutation in mouse[J]. CHINESE SCIENCE BULLETIN,1999,44(13):1207-1210.
APA	Li, SR.,Wang, DP.,Lan, H.,Zhang, WY.,Ge, BS.,...&Li, RF.(1999).Gene mapping of a new coat mutation in mouse.CHINESE SCIENCE BULLETIN,44(13),1207-1210.
MLA	Li, SR,et al."Gene mapping of a new coat mutation in mouse".CHINESE SCIENCE BULLETIN 44.13(1999):1207-1210.