Preparative Separation and Purification of Rebaudioside A from Steviol Glycosides Using Mixed-Mode Macroporous Adsorption Resins

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Preparative Separation and Purification of Rebaudioside A from Steviol Glycosides Using Mixed-Mode Macroporous Adsorption Resins
	Di DL(邸多隆); Liu YF(刘永峰); Di DL(邸多隆)
刊名	Journal of Agricultural and Food Chemistry
	2011
卷号	59 页码:9629-9636
关键词	Rebaudioside A mixed-mode macroporous adsorption resin steviol glycoside adsorption kinetics adsorption isotherms purification
ISSN号	0021-8561
通讯作者	邸多隆
中文摘要	Preparative separation and purification of rebaudioside A from steviol glycosides using mixed-mode macroporous adsorption resins (MARs) were systematically investigated. Mixed-mode MARs were prepared by a physical blending method. By evaluation of the adsorption/desorption ratio and adsorption/desorption capacity of mixed-mode MARs with different proportions toward RA and ST, the mixed-mode MAR 18 was chosen as the optimum strategy. On the basis of the static tests, it was found that the experimental data fitted best to the pseudosecond-order kinetics and Temkin_Pyzhev isotherm. Furthermore, the dynamic adsorption/desorption experiments were performed on the mini column packed with mixed-mode MAR 18. After one run treatment, the purity of rebaudioside A in purified product increased from 40.77 to 60.53%, with a yield rate of 38.73% (W/W), and that in residual product decreased from 40.77 to 36.17%, with a recovery yield of 57.61% (W/W). The total recovery yield reached 96.34% (W/W). The results showed that this method could be utilized in large-scale production of rebaudioside A from steviol glycosides in industry.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the National Natural Sciences Foundation of China (NSFC No. 20974116);the “Hundred Talents Program” of the Chinese Academy of Sciences (CAS)
语种	英语
WOS记录号	WOS:000294585100072
公开日期	2012-10-18
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/1554]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Di DL(邸多隆); Di DL(邸多隆)
推荐引用方式 GB/T 7714	Di DL,Liu YF,Di DL. Preparative Separation and Purification of Rebaudioside A from Steviol Glycosides Using Mixed-Mode Macroporous Adsorption Resins[J]. Journal of Agricultural and Food Chemistry,2011,59:9629-9636.
APA	邸多隆,刘永峰,&邸多隆.(2011).Preparative Separation and Purification of Rebaudioside A from Steviol Glycosides Using Mixed-Mode Macroporous Adsorption Resins.Journal of Agricultural and Food Chemistry,59,9629-9636.
MLA	邸多隆,et al."Preparative Separation and Purification of Rebaudioside A from Steviol Glycosides Using Mixed-Mode Macroporous Adsorption Resins".Journal of Agricultural and Food Chemistry 59(2011):9629-9636.