Glucose-induced Ca2+ signals in rat pancreatic beta cells

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	Glucose-induced Ca2+ signals in rat pancreatic beta cells
	Lou, XL ; Zeng, XH ; Qu, AL ; Zhou, Z
刊名	PROGRESS IN NATURAL SCIENCE
	2001
卷号	11 期号:2 页码:109-114
关键词	rat pancreatic beta cell glucose calcium oscillation calcium stores SECRETION DECREASE
ISSN号	1002-0071
通讯作者	Zhou, Z (reprint author), Huazhong Univ Sci & Technol, Inst Biophys & Biochem, Wuhan 430074, Peoples R China,
英文摘要	Using microfluorometry to assay intracellular Ca2+, the influences of varied factors on glucose-induced Ca2+ signals, such as glucose-induced initial decline phase (GIDP), Ca2+ oscillation, and Ca2+ release from internal stores, were investigated in single rat pancreatic beta cells. Glucose was able to evoke GIDP even at non-stimulus concentration (5 mol/L), which is insufficient to induce Ca2+ spikes. GIDP was dependent on neither membrane depolarization nor extracellular Ca2+. However, GIDP was inhibited by thapsigargin, indicating a dependence on Ca2+ uptake by Ca2+ stores. The glucose-induced calcium oscillation was inhibited when external Ca2+ was removed. However, thapsigargin could not block the Ca2+ oscillation. These results suggest that maintenance of Ca2+ oscillation requires extracellular Ca2+ but not Ca2+ stores. Glucose was able to evoke Ca2+ signals even in the absence of external Ca2+. The glucose-induced Ca2+ release from intracellular Ca2+ stores was blocked by TTX. However, TTX had no effect on high K+-induced Ca2+ store release, suggesting that membrane depolarization can directly release Ca2+ from some internal Ca2+ stores in beta cells.
学科主题	Materials Science ; Science & Technology - Other Topics
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/2080]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Lou, XL,Zeng, XH,Qu, AL,et al. Glucose-induced Ca2+ signals in rat pancreatic beta cells[J]. PROGRESS IN NATURAL SCIENCE,2001,11(2):109-114.
APA	Lou, XL,Zeng, XH,Qu, AL,&Zhou, Z.(2001).Glucose-induced Ca2+ signals in rat pancreatic beta cells.PROGRESS IN NATURAL SCIENCE,11(2),109-114.
MLA	Lou, XL,et al."Glucose-induced Ca2+ signals in rat pancreatic beta cells".PROGRESS IN NATURAL SCIENCE 11.2(2001):109-114.