Time course of action of antagonists of mitochondrial Ca uptake in intact ventricular myocytes

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	Time course of action of antagonists of mitochondrial Ca uptake in intact ventricular myocytes
	Zhou, Z ; Bers, DM
刊名	PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
	2002
卷号	445 期号:1 页码:132-138
关键词	Ca uptake FCCP ruthenium red biosensor Ru360 CCCP Indo-1 cardiac cells ADRENAL CHROMAFFIN CELLS CARDIAC MYOCYTES OSCILLATIONS COMPLEX
ISSN号	0031-6768
通讯作者	Zhou, Z (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China,
英文摘要	Mitochondrial Ca uptake is important for ATP production and intracellular Ca buffering. Several agents are often used to interfere with mitochondrial Ca transport, but the use of these agents in intact cells is complicated by lack of knowledge about how rapidly these agents gain access to intracellular mitochondria. We have developed a novel method to assess the time course with which extracellularly applied inhibitors gain access to mitochondria in intact ventricular myocytes preloaded with the fluorescent Ca indicator indo-1-AM. Cell contraction (assessed as the normalized change in cell length DeltaL(n)) was employed as an index of the cytosolic [Ca] ([Ca](c)), and was compared with the indo-1 ratio R-n. which reflects both mitochondrial [Ca] ([Ca](m)) and [Ca](c). Upon abrupt plasma membrane damage in control cells, the delay (t(k)) between the rise in L-n and R-n was < 10 s (reflecting the time lag between the change in [Ca]c and that in [Ca](m)). Exposure of cells to 50 muM ruthenium red (RR) increased t(k) as a monotonic function of preincubation time. In contrast, 10 muM Ru360, a selective and more potent Ca uniporter blocker (K-i similar to0.2 nM) reached a comparable maximal tk after only 10 min, making it practical to use in intact cells. Carbonylcyanide p(trifluoromethoxy)phenylhydrazone (FCCP) and carbonylcyanide m-chlorophenylhydrazone (CCCP) produced smaller maximal effects on tk, but did so almost immediately. These results are the first quantitative data on the time course of blockade of mitochondrial Ca uptake by the four most widely used mitochondrial Ca uptake antagonists in single ventricular myocytes.
学科主题	Physiology
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/2041]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Zhou, Z,Bers, DM. Time course of action of antagonists of mitochondrial Ca uptake in intact ventricular myocytes[J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY,2002,445(1):132-138.
APA	Zhou, Z,&Bers, DM.(2002).Time course of action of antagonists of mitochondrial Ca uptake in intact ventricular myocytes.PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY,445(1),132-138.
MLA	Zhou, Z,et al."Time course of action of antagonists of mitochondrial Ca uptake in intact ventricular myocytes".PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 445.1(2002):132-138.