Neuronal transmission stimulates the phosphorylation of Kv1.4 channel at Ser229 through protein kinase A1

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	Neuronal transmission stimulates the phosphorylation of Kv1.4 channel at Ser229 through protein kinase A1
	Tao, YM ; Zeng, R ; Shen, B ; Jia, JM ; Wang, YZ
刊名	JOURNAL OF NEUROCHEMISTRY
	2005
卷号	94 期号:6 页码:1512-1522
关键词	neurotransmission N-methyl-D-aspartate receptor phosphorylation protein kinase A Ser229 voltage-gated K+ channel 1.4 GATED POTASSIUM CHANNELS RAT HIPPOCAMPAL-NEURONS CANNABINOID RECEPTOR MODULATION METHYL-D-ASPARTATE K+ CHANNELS SYNAPTIC PLASTICITY STRIATAL NEURONS ALPHA-SUBUNITS IMMUNOHISTOCHEMICAL LOCALIZATION PHOSPHATIDYLINOSITOL 3-KINASE
ISSN号	0022-3042
通讯作者	Wang, YZ (reprint author), Chinese Acad Sci, Inst Neurosci, SIBS, Grad Sch, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,yzwang@ion.ac.cn
英文摘要	Phosphorylation of voltage- gated K+ channels (Kv) is involved in regulation of neuronal excitability, synaptic plasticity and neuronal survival. Among Kv channels expressed in the CNS, Kv1.4 is located in the soma, dendrite and axon terminus of neurones in most regions of the brain. Here, we show that Ser229 found within the highly conserved T1 domain of Kv1.4 in cultured rat cortical neurones is phosphorylated by protein kinase A (PKA), as demonstrated by in vitro protein kinase assay and Western blotting with a polyclonal antibody specific against phosphorylated Ser229. Glutamate, high concentrations of K+ or K+ channel blockers known to increase neurotransmission all stimulated the phosphorylation of Kv1.4 at Ser229 via N-methyl-D-aspartate (NMDA), but not alpha-amino-3-hydroxy-5-methylisoxazole-4-proprionic acid (AMPA) receptor, whereas tetradotoxin (TTX), known to block neuronal transmission, and depletion of extracellular Ca2+ inhibited phosphorylation induced by tetraethylammonium ( TEA), a nonselective K+ channel blocker. Mutation of Ser229 to Ala229 enhanced the current density. Taken together, elevation of the neuronal transmission stimulates the phosphorylation of Kv1.4 at Ser229 via the Ca2+ influx through NMDA receptor. Thus, it is possible that neuronal transmission regulates neuronal excitability partially through the phosphorylation of Kv1.4S229.
学科主题	Biochemistry & Molecular Biology ; Neurosciences & Neurology
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1885]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Tao, YM,Zeng, R,Shen, B,et al. Neuronal transmission stimulates the phosphorylation of Kv1.4 channel at Ser229 through protein kinase A1[J]. JOURNAL OF NEUROCHEMISTRY,2005,94(6):1512-1522.
APA	Tao, YM,Zeng, R,Shen, B,Jia, JM,&Wang, YZ.(2005).Neuronal transmission stimulates the phosphorylation of Kv1.4 channel at Ser229 through protein kinase A1.JOURNAL OF NEUROCHEMISTRY,94(6),1512-1522.
MLA	Tao, YM,et al."Neuronal transmission stimulates the phosphorylation of Kv1.4 channel at Ser229 through protein kinase A1".JOURNAL OF NEUROCHEMISTRY 94.6(2005):1512-1522.