N-methyl-D-aspartate receptor-dependent long-term potentiation in CA1 region affects synaptic expression of glutamate receptor subunits and associated proteins in the whole hippocampus

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	N-methyl-D-aspartate receptor-dependent long-term potentiation in CA1 region affects synaptic expression of glutamate receptor subunits and associated proteins in the whole hippocampus
	Zhong, W. X. ; Dong, Z. F. ; Tian, M. ; Cao, J. ; Xu, L. ; Luo, J. H.
刊名	NEUROSCIENCE
	2006
卷号	141 期号:3 页码:1399-1413
关键词	LTP glutamate receptors phosphorylation CaMKII alpha synaptosome AMPA RECEPTORS POSTSYNAPTIC DENSITY RAT HIPPOCAMPUS EXCITATORY SYNAPSES SURFACE EXPRESSION NMDA RECEPTORS ACID RECEPTOR GLUR1 SUBUNIT PLASTICITY TRAFFICKING
ISSN号	0306-4522
通讯作者	Luo, JH (reprint author), Zhejiang Univ, Sch Med, Dept Neurobiol, 353 Yanan Rd, Hangzhou 310006, Peoples R China,lxu@vip.163.com ; luojianhong@zjuem.zju.edu.cn
英文摘要	Long term potentiation in hippocampus, evoked by high-frequency stimulation, is mediated by two major glutamate receptor subtypes, alpha-amino-3-hydroxyl-5-methyl-4-isoxazole propionate receptors and N-methyl-D-aspartate receptors. Receptor subunit composition and its interaction with cytoplasmic proteins constitute different pathways regulating synaptic plasticity. Here, we provide further evidence that N-methyl-D-aspartate receptor-mediated long term potentiation evoked at hippocampal CA1 region of rats induced by high-frequency stimulation of the Schaffer collateral-commissural pathway in vivo is not dependent on N-methyl-D-aspartate receptor subunit NR2B. Applying semi-quantitative immunoblotting, we found that in the whole tetanized hippocampus, synaptic expression of the N-methyl-D-aspartate and a-amino-3-hydroxyl-5-methyl-4-isoxazole propionate receptor subunits (NR1, NR2A, glutamate receptor 1) and their associated partners, e.g. synaptic associated protein 97, postsynaptic density protein 95, a subunit of Ca(2+)/calmodulin-dependent protein kinase 11, neuronal nitricoxide synthase, increased 180 min post-high-frequency stimulation. Moreover, phosphorylation of Ca(2+)/calmodulin-dependent protein kinase II at thr286 and glutamate receptor 1 at ser831 was increased 30 min post-high-frequency stimulation and blocked by N-methyl-D-aspartate receptor antagonists (AP-5 and MK-801). In sham group and controls, these changes were not observed. The expression of several other synaptic proteins (NR2B, glutamate receptors 2/3, N-ethylmaleimide sensitive factor) was not affected by long term potentiation induction. In hippocampal homogenates, the level of these proteins remained unchanged. These data indicate that N-methyl-D-aspartate receptor-dependent long term potentiation in CA1 region in vivo mainly affects the synaptic expression of glutamate receptor subunits and associated proteins in the whole hippocampus. The alteration of molecular aspects can play a role in regulating the long-lasting synaptic modification in hippocampal long term potentiation in vivo. (c) 2006 IERO. Published by Elsevier Ltd. All rights reserved.
学科主题	Neurosciences & Neurology
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1850]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Zhong, W. X.,Dong, Z. F.,Tian, M.,et al. N-methyl-D-aspartate receptor-dependent long-term potentiation in CA1 region affects synaptic expression of glutamate receptor subunits and associated proteins in the whole hippocampus[J]. NEUROSCIENCE,2006,141(3):1399-1413.
APA	Zhong, W. X.,Dong, Z. F.,Tian, M.,Cao, J.,Xu, L.,&Luo, J. H..(2006).N-methyl-D-aspartate receptor-dependent long-term potentiation in CA1 region affects synaptic expression of glutamate receptor subunits and associated proteins in the whole hippocampus.NEUROSCIENCE,141(3),1399-1413.
MLA	Zhong, W. X.,et al."N-methyl-D-aspartate receptor-dependent long-term potentiation in CA1 region affects synaptic expression of glutamate receptor subunits and associated proteins in the whole hippocampus".NEUROSCIENCE 141.3(2006):1399-1413.