Syne-1 and Syne-2 play crucial roles in myonuclear anchorage and motor neuron innervation

CORC > 上海神经科学研究所 > 中国科学院上海生命科学研究院神经科学研究所 > 神经所(总)

	Syne-1 and Syne-2 play crucial roles in myonuclear anchorage and motor neuron innervation
	Zhang, Xiaochang ; Xu, Rener ; Zhu, Binggen ; Yang, Xiujuan ; Ding, Xu ; Duan, Shumin ; Xu, Tian ; Zhuang, Yuan ; Han, Min
刊名	DEVELOPMENT
	2007
卷号	134 期号:5 页码:901-908
关键词	synaptic nuclei neuromuscular junction neonatal lethality nuclear envelope KASH SUN domain nesprin ANC-1 MSP-300 mouse NEMATODE CAENORHABDITIS-ELEGANS NUCLEAR-MEMBRANE PROTEIN NEUROMUSCULAR-JUNCTION ACTIN CYTOSKELETON MUSCLE-FIBERS IN-VIVO DROSOPHILA ENVELOPE KLARSICHT GENE
ISSN号	0950-1991
通讯作者	Xu, RN (reprint author), Fudan Univ, Sch Life Sci, Inst Dev Biol & Mol Med, Shanghai 200433, Peoples R China,rener_xu@fudan.edu.cn
英文摘要	Proper nuclear positioning is important to cell function in many biological processes during animal development. In certain cells, the KASH-domain-containing proteins have been shown to be associated with the nuclear envelope, and to be involved in both nuclear anchorage and migration. We investigated the mechanism and function of nuclear anchorage in skeletal muscle cells by generating mice with single and double-disruption of the KASH-domain-containing genes Syne1 (also known as Syne-1) and Syne2 (also known as Syne-2). We showed that the deletion of the KASH domain of Syne-1 abolished the formation of clusters of synaptic nuclei and disrupted the organization of non-synaptic nuclei in skeletal muscle. Further analysis indicated that the loss of synaptic nuclei in Syne-1 KASH-knockout mice significantly affected the innervation sites and caused longer motor nerve branches. Although disruption of neither Syne-1 nor Syne-2 affected viability or fertility, Syne-1; Syne-2 double-knockout mice died of respiratory failure within 20 minutes of birth. These results suggest that the KASH-domain-containing proteins Syne-1 and Syne-2 play crucial roles in anchoring both synaptic and non-synaptic myonuclei that are important for proper motor neuron innervation and respiration.
学科主题	Developmental Biology
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1784]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Zhang, Xiaochang,Xu, Rener,Zhu, Binggen,et al. Syne-1 and Syne-2 play crucial roles in myonuclear anchorage and motor neuron innervation[J]. DEVELOPMENT,2007,134(5):901-908.
APA	Zhang, Xiaochang.,Xu, Rener.,Zhu, Binggen.,Yang, Xiujuan.,Ding, Xu.,...&Han, Min.(2007).Syne-1 and Syne-2 play crucial roles in myonuclear anchorage and motor neuron innervation.DEVELOPMENT,134(5),901-908.
MLA	Zhang, Xiaochang,et al."Syne-1 and Syne-2 play crucial roles in myonuclear anchorage and motor neuron innervation".DEVELOPMENT 134.5(2007):901-908.