A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons

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	A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons
	Wang, Zuoren
刊名	EUROPEAN JOURNAL OF NEUROSCIENCE
	2007
卷号	26 期号:9 页码:2405-2416
关键词	dopaminergic neuron giant gustatory neuron nociception painless MUSHROOM BODY SYNAPTIC-TRANSMISSION TASTE PERCEPTION ADULT DROSOPHILA EXCITABLE CELLS OPTICAL CONTROL MELANOGASTER ACTIVATION FIBER GENE
ISSN号	0953-816X
通讯作者	Zhang, W (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Neurosci, Inst Neurosci, Shanghai 200031, Peoples R China,ZuorenWang@ion.ac.cn
英文摘要	In order to study the function of specific neural circuits, we generated UAS-Channelrhodopsin2 (ChR2) transgenic Drosophila and established a ChR2-based system that enables specific activation of targeted neurons in larval and adult fruit flies with blue light illumination, under the control of a newly designed light source that provides fully programmable stimulation patterns. We showed that stimulating selectively the nociceptor of larvae expressing ChR2 elicited light-induced 'pain' response, confined freely behaving larvae in defined area and directed larva migration along a preset route. In freely behaving adult flies, rapid photoactivation of targeted gustatory sensory neurons, dopaminergic modulatory neurons and motor neurons triggered the proboscis extension response, escaping reflex and changes in the locomotion pattern, respectively, with precise temporal control. This non-invasive method for remote control of animal behaviors also provides a potential tool for conducting 'gain of function' studies toward understanding how animal behaviors are controlled by neural activity.
学科主题	Neurosciences & Neurology
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1749]
专题	上海神经科学研究所_神经所(总) 上海神经科学研究所_神经环路与动物行为研究组
推荐引用方式 GB/T 7714	Wang, Zuoren. A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons[J]. EUROPEAN JOURNAL OF NEUROSCIENCE,2007,26(9):2405-2416.
APA	Wang, Zuoren.(2007).A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons.EUROPEAN JOURNAL OF NEUROSCIENCE,26(9),2405-2416.
MLA	Wang, Zuoren."A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons".EUROPEAN JOURNAL OF NEUROSCIENCE 26.9(2007):2405-2416.