Fluoxetine potentiates GABAergic IPSCs in rat hippocampal neurons

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	Fluoxetine potentiates GABAergic IPSCs in rat hippocampal neurons
	Ye, Zeng-You ; Zhou, Ke-Qing ; Xu, Tian-Le(徐天乐) ; Zhou, Jiang-Ning
刊名	NEUROSCIENCE LETTERS
	2008
卷号	442 期号:1 页码:24-29
关键词	GABAergic hypothesis whole-cell patch-clamp recording antidepressant neurotransmission 5-HT GAMMA-AMINOBUTYRIC-ACID MAGNETIC-RESONANCE-SPECTROSCOPY DEPRESSED-PATIENTS PLASMA-CONCENTRATIONS AFFECTIVE-ILLNESS MAJOR DEPRESSION MOOD DISORDERS GABA SEROTONIN NORFLUOXETINE
ISSN号	0304-3940
通讯作者	Zhou, JN (reprint author), Univ Sci & Technol China, Hefei Natl Lab Phys Sci & Microscale, Hefei 230027, Anhui, Peoples R China,zyye@mail.ustc.edu.cn
英文摘要	The GABA system is highly involved in the pathophysiology of mood disorders such as depression. Altered GABAergic function is evident in depressed patients and animal models of depression. Currently, the most widely used antidepressants are selective 5-HT reuptake inhibitors, such as fluoxetine. However, the effects of fluoxetine on GABAergic synaptic neurotransmission remain poorly investigated. Whole-cell patch-clamp recordings from cultured rat hippocampal neurons were therefore conducted to investigate the effects of fluoxetine on GABAergic neurotransmission. The spontaneous inhibitory postsynaptic current (sIPSC) was completely blocked by 10 mu M bicuculline and reversibly potentiated by 30 mu M fluoxetine. The fluoxetine potentiation on either amplitude or frequency of sIPSCs was dose-dependent, with the EC50 values of 10.96 and 14.26 mu M, respectively. This potentiation was also TTX-insensitive, suggesting independence of presynaptic action potentials. The ritanserin (5 mu M), a selective 5-HT2 receptor antagonist, did not alter the fluoxetine potentiation on miniature inhibitory postsynaptic currents. Taken together, our data suggest that fluoxetine can potentiate GABAergic neurotransmission without depending on presynaptic firing of action potentials and its elevating of 5-HT receptor activities. This potentiation by fluoxetine may normalize the hippocampal GABA deficit during depression and in part exert its antidepressant activity. (c) 2008 Elsevier Ireland Ltd. All rights reserved.
学科主题	Neurosciences & Neurology
收录类别	SCI
资助信息	National Key Project for Basic Science of China[2006CB500705]; Natural Science Foundation of China[30530310]
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1701]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Ye, Zeng-You,Zhou, Ke-Qing,Xu, Tian-Le,et al. Fluoxetine potentiates GABAergic IPSCs in rat hippocampal neurons[J]. NEUROSCIENCE LETTERS,2008,442(1):24-29.
APA	Ye, Zeng-You,Zhou, Ke-Qing,Xu, Tian-Le,&Zhou, Jiang-Ning.(2008).Fluoxetine potentiates GABAergic IPSCs in rat hippocampal neurons.NEUROSCIENCE LETTERS,442(1),24-29.
MLA	Ye, Zeng-You,et al."Fluoxetine potentiates GABAergic IPSCs in rat hippocampal neurons".NEUROSCIENCE LETTERS 442.1(2008):24-29.