PKC delta regulates cortical radial migration by stabilizing the Cdk5 activator p35

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	PKC delta regulates cortical radial migration by stabilizing the Cdk5 activator p35
	Zhao, Chun-tao ; Li, Kun ; Li, Jun-tao ; Zheng, Wang ; Liang, Xu-jun ; Geng, An-qi ; Li, Ning ; Yuan, Xiao-bing
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2009
卷号	106 期号:50 页码:21353-21358
关键词	BDNF neuronal migration phosphorylation protein stability ubiquitin PROTEIN-KINASE-C CYCLIN-DEPENDENT KINASE-5 NEURONAL-SPECIFIC ACTIVATOR DEVELOPING CEREBRAL-CORTEX CELL-MIGRATION UP-REGULATION MECHANISMS NEOCORTEX GENE PHOSPHORYLATION
ISSN号	0027-8424
通讯作者	Yuan, XB (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai 200031, Peoples R China,yuanxb@ion.ac.cn
英文摘要	Cyclin-dependent kinase 5 (Cdk5) and its activator p35 are critical for radial migration and lamination of cortical neurons. However, how this kinase is regulated by extracellular and intracellular signals during cortical morphogenesis remains unclear. Here, we show that PKC delta, a member of novel PKC expressing in cortical neurons, could stabilize p35 by direct phosphorylation. PKC delta attenuated the degradation of p35 but not its mutant derivative, which could not be phosphorylated by PKC delta. Down-regulation of PKC delta by in utero electroporation of specific small interference RNA (siRNA) severely impaired the radial migration of cortical neurons. This migration defect was similar to that caused by down-regulation of p35 and could be prevented by cotransfection with the wild-type but not the mutant p35. Furthermore, PKC delta could be activated by the promigratory factor brain-derived neurotrophic factor (BDNF) and was required for the activation of Cdk5 by BDNF. Both PKC delta and p35 were required for the promigratory effect of BDNF on cultured newborn neurons. Thus, PKC delta may promote cortical radial migration through maintaining the proper level of p35 in newborn neurons.
学科主题	Science & Technology - Other Topics
收录类别	SCI
资助信息	973 Project[2006CB806600, 2006CB943903]; National Natural Science Foundation of China[30625023, 30721004]; Chinese Academy of Sciences[KSCX2-YW-R-103]
语种	英语
公开日期	2012-07-13
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1625]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Zhao, Chun-tao,Li, Kun,Li, Jun-tao,et al. PKC delta regulates cortical radial migration by stabilizing the Cdk5 activator p35[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2009,106(50):21353-21358.
APA	Zhao, Chun-tao.,Li, Kun.,Li, Jun-tao.,Zheng, Wang.,Liang, Xu-jun.,...&Yuan, Xiao-bing.(2009).PKC delta regulates cortical radial migration by stabilizing the Cdk5 activator p35.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,106(50),21353-21358.
MLA	Zhao, Chun-tao,et al."PKC delta regulates cortical radial migration by stabilizing the Cdk5 activator p35".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 106.50(2009):21353-21358.