Fluorescent Labeling of Newborn Dentate Granule Cells in GAD67-GFP Transgenic Mice: A Genetic Tool for the Study of Adult Neurogenesis

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	Fluorescent Labeling of Newborn Dentate Granule Cells in GAD67-GFP Transgenic Mice: A Genetic Tool for the Study of Adult Neurogenesis
	Zhao, Shengli ; Zhou, Yang ; Gross, Jimmy ; Miao, Pei ; Qiu, Li ; Wang, Dongqing ; Chen, Qian ; Feng, Guoping
刊名	PLOS ONE
	2010
卷号	5 期号:9 页码:e12506-e12507
关键词	NEURAL STEM-CELLS CENTRAL-NERVOUS-SYSTEM HIPPOCAMPAL NEUROGENESIS SYNAPTIC PLASTICITY GLUTAMIC-ACID FUNCTIONAL IMPLICATIONS GABAERGIC PHENOTYPE SUBVENTRICULAR ZONE GYRUS NEURONS
ISSN号	1932-6203
通讯作者	Zhao, SL (reprint author), Duke Univ, Med Ctr, Dept Neurobiol, Durham, NC 27710 USA,feng@neuro.duke.edu
英文摘要	Neurogenesis in the adult hippocampus is an important form of structural plasticity in the brain. Here we report a line of BAC transgenic mice (GAD67-GFP mice) that selectively and transitorily express GFP in newborn dentate granule cells of the adult hippocampus. These GFP+ cells show a high degree of colocalization with BrdU-labeled nuclei one week after BrdU injection and express the newborn neuron marker doublecortin and PSA-NCAM. Compared to mature dentate granule cells, these newborn neurons show immature morphological features: dendritic beading, fewer dendritic branches and spines. These GFP+ newborn neurons also show immature electrophysiological properties: higher input resistance, more depolarized resting membrane potentials, small and non-typical action potentials. The bright labeling of newborn neurons with GFP makes it possible to visualize the details of dendrites, which reach the outer edge of the molecular layer, and their axon (mossy fiber) terminals, which project to the CA3 region where they form synaptic boutons. GFP expression covers the whole developmental stage of newborn neurons, beginning within the first week of cell division and disappearing as newborn neurons mature, about 4 weeks postmitotic. Thus, the GAD67-GFP transgenic mice provide a useful genetic tool for studying the development and regulation of newborn dentate granule cells.
学科主题	Life Sciences & Biomedicine - Other Topics
收录类别	SCI
资助信息	Arnold and Mabel Beckman Foundation
语种	英语
公开日期	2012-07-13
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1576]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Zhao, Shengli,Zhou, Yang,Gross, Jimmy,et al. Fluorescent Labeling of Newborn Dentate Granule Cells in GAD67-GFP Transgenic Mice: A Genetic Tool for the Study of Adult Neurogenesis[J]. PLOS ONE,2010,5(9):e12506-e12507.
APA	Zhao, Shengli.,Zhou, Yang.,Gross, Jimmy.,Miao, Pei.,Qiu, Li.,...&Feng, Guoping.(2010).Fluorescent Labeling of Newborn Dentate Granule Cells in GAD67-GFP Transgenic Mice: A Genetic Tool for the Study of Adult Neurogenesis.PLOS ONE,5(9),e12506-e12507.
MLA	Zhao, Shengli,et al."Fluorescent Labeling of Newborn Dentate Granule Cells in GAD67-GFP Transgenic Mice: A Genetic Tool for the Study of Adult Neurogenesis".PLOS ONE 5.9(2010):e12506-e12507.