Tetranectin and apolipoprotein A-I in cerebrospinal fluid as potential biomarkers for Parkinson's disease

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	Tetranectin and apolipoprotein A-I in cerebrospinal fluid as potential biomarkers for Parkinson's disease
	Wang, E. -S. ; Sun, Y. ; Guo, J. -G. ; Gao, X. ; Hu, J. -W. ; Zhou, L. ; Hu, J. ; Jiang, C. -C.
刊名	ACTA NEUROLOGICA SCANDINAVICA
	2010
卷号	122 期号:5 页码:350-359
关键词	Parkinson's disease cerebrospinal fluid biomarker proteomics DIFFERENCE GEL-ELECTROPHORESIS ALZHEIMERS-DISEASE MULTIPLE-SCLEROSIS PROTEOMIC ANALYSIS NEURODEGENERATIVE DISEASES PROTEIN MARKERS 2-D DIGE CSF BRAIN EXPRESSION
ISSN号	0001-6314
通讯作者	Jiang, CC (reprint author), Fudan Univ, Huashan Hosp, Dept Neurosurg, 12 Wulumuqi Middle Rd, Shanghai 200040, Peoples R China,wesong@sina.com
英文摘要	Objective - The application of biomarkers may potentially improve the efficiency of the diagnosis for Parkinson's disease (PD). However, no reliable biomarker has been identified to date. This study is aimed to identify proteins that might serve as potential biomarkers for PD diagnosis or pathogenesis. Materials and methods - Two-dimensional difference gel electrophoresis (2D DIGE) technique, in combination with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS), was used to determine the differentially expressed cerebrospinal fluid (CSF) proteins in PD patients (n = 3) compared with normal controls (n = 3). Selected proteins were further confirmed by Western blotting analysis in the CSF of PD patients (n = 8), Alzheimer's disease (AD) patients (n = 6) and normal control subjects (n = 7). Results - Eight proteins were identified after MS and protein database interrogation. In the CSF of PD patients, the expression levels of one isoform of apolipoprotein A-I (apoA-I), tetranectin, myosin phosphatase target subunit 1 (MYPT1), and two unknown proteins were down-regulated, whereas the expression levels of another apoA-I isoform, proapolipoprotein, and lipoprotein were up-regulated. Western blotting indicates that the expression of tetranectin was reduced in the CSF from PD patients and elevated in AD, while the expression of apoA-I was changed only in the CSF from PD patients. Conclusion - Our preliminary results suggest that tetranectin and apoA-I may serve as potential biomarkers for PD, though further validation is needed.
学科主题	Neurosciences & Neurology
收录类别	SCI
资助信息	Shanghai Metropolitan Fund for Research and Development[04dz14005]; Shanghai Pujiang Program
语种	英语
公开日期	2012-07-13
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1567]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Wang, E. -S.,Sun, Y.,Guo, J. -G.,et al. Tetranectin and apolipoprotein A-I in cerebrospinal fluid as potential biomarkers for Parkinson's disease[J]. ACTA NEUROLOGICA SCANDINAVICA,2010,122(5):350-359.
APA	Wang, E. -S..,Sun, Y..,Guo, J. -G..,Gao, X..,Hu, J. -W..,...&Jiang, C. -C..(2010).Tetranectin and apolipoprotein A-I in cerebrospinal fluid as potential biomarkers for Parkinson's disease.ACTA NEUROLOGICA SCANDINAVICA,122(5),350-359.
MLA	Wang, E. -S.,et al."Tetranectin and apolipoprotein A-I in cerebrospinal fluid as potential biomarkers for Parkinson's disease".ACTA NEUROLOGICA SCANDINAVICA 122.5(2010):350-359.